As already described, the complete DDRT-PCR analysis of two mammalian cell types, in duplicate, by using three one-base-anchored primers (H-dT11M) and 26 upstream random primers (H-APs) requires a total of 312 PCR reactions. Less than 3.5 μg total RNA is needed, and the amplified cDNAs can be analyzed on 13 sequencing gels, each consisting of 24 lanes.
KeywordsTitration MgCl2 Radionuclide Gelatin dNTP
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