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Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes

  • Mark Jackman
Part of the Springer Lab Manual book series (SLM)

Abstract

Many different proteins have now been expressed in insect cells using the baculoviral expression system. This system was developed using the Autographa californica nuclear polyhedrosis baculoviral strain (AcNPV). Baculovirus protein expression requires infection of insect cells with recombinant virus whose regions encoding for nonessential coat protein(s) have been replaced with a gene encoding for the protein required to be expressed.

Keywords

Insect Cell Partial Purification Transfer Vector Recombinant Baculovirus Baculoviral Expression System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Desai D, Gu Y, Morgan DO (1992). Activation of human cyclin-dependent kinases in vitro. Mol Biol Cell 3 571–582PubMedGoogle Scholar
  2. Hannon GJ (1995). Expression of cell cycle proteins using recombinant baculovirus. In: M. Pagano (ed) Cell cycle-Material and Methods. Springer, Berlin, Heidelberg, New York, pp 231–242Google Scholar
  3. O’Reilly D, Miller LK, Luckow VA (1992). Baculovirus expression vectors, a laboratory manual. W. H. Freeman New YorkGoogle Scholar
  4. Parker LL, Walter, SA, Young PG, Piwnica-Worms, H (1993). Phosphorylation and inactivation of the mitotic inhibitor Weel by the niml/cdrl kinase. Nature 363:736–738PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Mark Jackman
    • 1
  1. 1.Institute of Cancer and Developmental BiologyWellcome/CRC InstituteCambridgeUK

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