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Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes

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Animal Cell Culture Techniques

Part of the book series: Springer Lab Manual ((SLM))

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Abstract

Many different proteins have now been expressed in insect cells using the baculoviral expression system. This system was developed using the Autographa californica nuclear polyhedrosis baculoviral strain (AcNPV). Baculovirus protein expression requires infection of insect cells with recombinant virus whose regions encoding for nonessential coat protein(s) have been replaced with a gene encoding for the protein required to be expressed.

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© 1998 Springer-Verlag Berlin Heidelberg

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Jackman, M. (1998). Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes. In: Clynes, M. (eds) Animal Cell Culture Techniques. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80412-0_9

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  • DOI: https://doi.org/10.1007/978-3-642-80412-0_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-63008-1

  • Online ISBN: 978-3-642-80412-0

  • eBook Packages: Springer Book Archive

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