Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes

  • Mark Jackman
Part of the Springer Lab Manual book series (SLM)


Many different proteins have now been expressed in insect cells using the baculoviral expression system. This system was developed using the Autographa californica nuclear polyhedrosis baculoviral strain (AcNPV). Baculovirus protein expression requires infection of insect cells with recombinant virus whose regions encoding for nonessential coat protein(s) have been replaced with a gene encoding for the protein required to be expressed.


Insect Cell Partial Purification Transfer Vector Recombinant Baculovirus Baculoviral Expression System 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Mark Jackman
    • 1
  1. 1.Institute of Cancer and Developmental BiologyWellcome/CRC InstituteCambridgeUK

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