Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes
Many different proteins have now been expressed in insect cells using the baculoviral expression system. This system was developed using the Autographa californica nuclear polyhedrosis baculoviral strain (AcNPV). Baculovirus protein expression requires infection of insect cells with recombinant virus whose regions encoding for nonessential coat protein(s) have been replaced with a gene encoding for the protein required to be expressed.
KeywordsVortex Agarose HEPES Aeration Gentamicin
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- Hannon GJ (1995). Expression of cell cycle proteins using recombinant baculovirus. In: M. Pagano (ed) Cell cycle-Material and Methods. Springer, Berlin, Heidelberg, New York, pp 231–242Google Scholar
- O’Reilly D, Miller LK, Luckow VA (1992). Baculovirus expression vectors, a laboratory manual. W. H. Freeman New YorkGoogle Scholar