Primary Culture

  • Finbar O’Sullivan
  • Paula Meleady
  • Shirley McBride
  • Martin Clynes
Part of the Springer Lab Manual book series (SLM)


Primary culture involves the initial in vitro cultivation of cells obtained directly from animal tissue. While the cultivation of animal cells on serum clots had been performed since the early 1900s, it has really been in the last 20–30 years that significant progress has been made in the culture of specific differentiated cell types. Much information is available in the literature on the cultivation of specific cell types but is beyond the scope of this short chapter.


Primary Culture Sodium Selenite Normal Human Keratinocytes Antimitotic Agent Laminar Flow Cabinet 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Finbar O’Sullivan
    • 1
  • Paula Meleady
  • Shirley McBride
  • Martin Clynes
  1. 1.National Cell and Tissue Culture Center, Dublin City UniversityGlasnevin, Dublin 9Ireland

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