Primary culture involves the initial in vitro cultivation of cells obtained directly from animal tissue. While the cultivation of animal cells on serum clots had been performed since the early 1900s, it has really been in the last 20–30 years that significant progress has been made in the culture of specific differentiated cell types. Much information is available in the literature on the cultivation of specific cell types but is beyond the scope of this short chapter.
KeywordsHydrocortisone Pyruvate Trypsin Estradiol Dextran
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