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Primary Culture

  • Finbar O’Sullivan
  • Paula Meleady
  • Shirley McBride
  • Martin Clynes
Part of the Springer Lab Manual book series (SLM)

Abstract

Primary culture involves the initial in vitro cultivation of cells obtained directly from animal tissue. While the cultivation of animal cells on serum clots had been performed since the early 1900s, it has really been in the last 20–30 years that significant progress has been made in the culture of specific differentiated cell types. Much information is available in the literature on the cultivation of specific cell types but is beyond the scope of this short chapter.

Keywords

Primary Culture Sodium Selenite Normal Human Keratinocytes Antimitotic Agent Laminar Flow Cabinet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baron J, Voigt JM (1990) Localisation, distribution and induction of xenobioticmetabolising enzymes and aryl hydrocarbon hyroxylase activity within lung. Pharmac Ther 47:419–445CrossRefGoogle Scholar
  2. Bower M, Carney DH, Oie Hk, Gazdar AF, Minna JD (1986) Growth of cell lines and clinical specimens of human non-small cell cancer in a serum-free defined medium. Cancer Res 46:798–806Google Scholar
  3. Carney DN, Bunn PA, Gazdar, AF, Pagan JF, and Minna, JD (1981) Selective growth in serum free hormone supplemented medium of tumor cells obtained by biopsy from patients with small cell carcinoma of the lung Proc Natl Acad Sci USA 78: 3185–3189PubMedCrossRefGoogle Scholar
  4. Cunningham AC, Milne DS, Wilkes J, Dark JH, Tetley TD and Kirby JA (1994) Constitutive expression of MHC and adhesion molecules by alveolar epithelial cells (type II pneumonocytes) isolated from human lung and comparison with immunocytochemical findings. J Cell Sci 107:443–449PubMedGoogle Scholar
  5. Devereux TR, Massey TE, Van Scott MR, Yankaskas J and Fouts JR (1986) Xenobiotic metabolism in human alveolar type II cells isolated by centrifugal elutriation and density gradient centrifugation. Can Res 46:5438–5443Google Scholar
  6. Dichter MA (1987) Rat cortical neurons in cell culture: culture methods, cell morphology, electrophysiology, and synapse formation. Brian Research 149: 279–293CrossRefGoogle Scholar
  7. Ebendahl T (1979) Stage-dependent stimulation of neurite outgrowth exerted by nerve growth factor and chick heart in cultured embryonic ganglia. Dev Biol 72: 276CrossRefGoogle Scholar
  8. El-Badry OM (1991) Insulin-like growth factor II gene expression in human neuroblastoma. Adv Neuroblastoma Res 3: 249–256Google Scholar
  9. Freshney RI (1994) Culture of animal cells: a manual of basic technique, 3rd edition. Alan R Liss, New YorkGoogle Scholar
  10. Gazar AF, Linnolia RI, Kurita Y, Oie HK, Mulsine JL, Clark JC, Whistsett JA(1990) Peripheral airway differentiation in human lung cancer cell lines. Cancer Res 50: 5481–5487Google Scholar
  11. Grizzle,WE, Polt SS (1998) Guidelines to avoid personnel contamination by invective agents in research laboratories that use human tissues. J Tissue Culture Methods 11:191–199CrossRefGoogle Scholar
  12. Ham RG, McKeehan WL (1979) Media and growth requirements. Methods Enzymol LVII:44–93CrossRefGoogle Scholar
  13. Kikkawa Y, Yoneda K (1974) The type II epithelial cells of the lung, 1: methods of isolation. Lab Invest 30:76–84PubMedGoogle Scholar
  14. Klein JC, Zurcher C., van Bekkum DW. (1987) Differential behaviour of human bronchial carcinoma cells in culture Cancer Res 47: 3251–3258PubMedGoogle Scholar
  15. Lebskowski JS, Schair LR, and Okarma TB (1985) Serum free culture of hematopoietic stem cells: a review. Stem Cells 13:607–612CrossRefGoogle Scholar
  16. Sandstrom CE, Miller WM, Papoutsakis ET (1994) Review: Serum-free media for cultures of primitive and mature hematopoietic cells. Biotechnol Bioeng 43:706–733PubMedCrossRefGoogle Scholar
  17. Liu S , Mautone AJ (1996) Whole cell potassium currents in fetal rat alveolar type II epithelial cells cultured on Matrigel matrix. Am J Physiol 270:L577–L586PubMedGoogle Scholar
  18. MassonEA, Atkin SL, White MC (1993) D-valine selective medium does not inhibit human fibroblast growth in vitro. In Vitro Cell Dev Biol 29A: 912–913CrossRefGoogle Scholar
  19. Oie HK, Russelle EK, Carney DN, Gazdar AF (1996) Cell culture methods for the establishment of the NCI series of lung cancer cells J Cell Biochem Sup 24: 24–31CrossRefGoogle Scholar
  20. Rahemtulla F, Moorer CM and Wille Jr. JJ (1989) Biosynthesis of proteoglycans by proliferating and differentiating normal human keratinocytes cultured in serum free medium. J Cell Physiol 140: 98–106.PubMedCrossRefGoogle Scholar
  21. Resnicoff M., Medrano EE, Podhajcer OL, Bravo AI, Bouer L, Mordoh J (1987) Subpopulations of MCF-7 cells seperated by percoll gradient centrifugation a model to analyze the heterogeneity of human breast cancer. Proc Natl Acad Sci USA 84:7295–7294PubMedCrossRefGoogle Scholar
  22. Viscardi RM, Ullsperger S, Resau JH (1992) Reproducible isolation of type II pneumocytes from fetal and adult lung using Nycodenz density gradients. Exp Lung Res 18:225–245PubMedCrossRefGoogle Scholar
  23. Yoshida T, Takeuchi M (1991) Primary culture and cyropreservation of mouse astrocytes under serum-free conditions. Cytotechnology 5: 99–106PubMedCrossRefGoogle Scholar
  24. Yavin Z,Yavin E (1980) Survival and maturation of cerebral neurons on poly (L-lysine) surfaces in the absence of serum. Dev Biol 75: 454–460.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Finbar O’Sullivan
    • 1
  • Paula Meleady
  • Shirley McBride
  • Martin Clynes
  1. 1.National Cell and Tissue Culture Center, Dublin City UniversityGlasnevin, Dublin 9Ireland

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