Abstract
The study of gene regulation has expanded enormously over the last 20 years due to the availability of a wide range of new techniques. It is now central to molecular biology and is essential to an understanding of any gene or protein. Initially it was believed that transcription was driven by the interaction of transcription factors with conserved cis-elements in gene promoters. However, with the discovery of enhancer sequences and transcription factor binding sites within introns it was realized that gene transcription was a far more complex process. Gene expression is controlled (in part) by the binding of two classes of transcription factors to conserved regulatory elements.
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References
Bellard M, Dretzen G, Giangrande A, Ramain P (1989) Nuclease digestion of transcriptionally active chromatin. In: Wasserman PM, Kornberg RD (eds) Nucleosomes. Methods Enzymol 179:317–346
Bossard P, Parsa R, Decaux J-F, Iynedjian P, Girard J (1993) Glucose administration induces the premature expression of liver glucokinase gene in newborn rats. Eur J Biochem 215:883–892
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Greenberg ME, Bender TP (1989) Identification of newly transcribed RNA. Nuclear runoff transcription in mammalian cells. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. Wiley, Chichester, pp 4.10.1–4.10.9
Marzluff MF, Huang RCC (1985) Transcription of RNA in isolated nuclei. In: Hames BD, Higgins SJ (eds) Transcription and translation: a practical approach. IRL, Oxford, pp 89–129
Neznanov NS, Oshima RG (1993) Cis regulation of the keratin 18 gene in transgenic mice. Mol Cell Biol 13:1815–1823
Parsa R, Decaux J-F, Bossard P, Robey BR, Magnuson MA, Granner DK, Girard J (1996) Induction of the glucokinase gene by insulin in cultured neonatal rat hepatocytes. Relationship with DNase-I hypersensitive sites and functional analysis of a putative insulin-response element. Eur J Biochem 236:214–221
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, New York
Stewart AF, Reik A, Schutz G (1991) A simpler and better method to cleave chromatin with DNase I for hypersensitive site analyses. Nucleic Acids Res 19:3157.
Wu C (1989) Analysis of hypersensitive sites in chromatin. In: Wasserman PM, Romberg RD (eds) Nucleosomes. Methods Enzymol 170:269–289
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© 1998 Springer-Verlag Berlin Heidelberg
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Daly, N., Clynes, M. (1998). Methods for Studying the Transcription and DNase I Hypersensitive Sites of Genes in Cells in Culture. In: Clynes, M. (eds) Animal Cell Culture Techniques. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80412-0_31
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DOI: https://doi.org/10.1007/978-3-642-80412-0_31
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-63008-1
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