Methods for Studying the Transcription and DNase I Hypersensitive Sites of Genes in Cells in Culture
The study of gene regulation has expanded enormously over the last 20 years due to the availability of a wide range of new techniques. It is now central to molecular biology and is essential to an understanding of any gene or protein. Initially it was believed that transcription was driven by the interaction of transcription factors with conserved cis-elements in gene promoters. However, with the discovery of enhancer sequences and transcription factor binding sites within introns it was realized that gene transcription was a far more complex process. Gene expression is controlled (in part) by the binding of two classes of transcription factors to conserved regulatory elements.
KeywordsNorthern Blot Analysis Enhancer Sequence Hypersensitive Site Hybridization Temperature Random Primer Label
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