Cytokine Cell Culture Assays

  • Alice Redmond
  • Jacqueline Quinn
  • Susan Molloy
Part of the Springer Lab Manual book series (SLM)


Cytokines are defined as secreted regulatory proteins that control the survival, growth, differentiation and effector function of tissues. Cytokines encompass those families of regulators known as growth factors, colony-stimulating factors, interleukins (ILs), lymphokines, monokines and interferons (IFNs). Measuring cytokines is an important facet of understanding the complex interactions of the immune response mediated by these multifunctional, peptide, T cell products. For example cytokines regulate the activation, growth and differentiation of many cell types and have been implicated in diseases such as arthritis, allergy and leukemia (Aria 1990). In some cases, cytokines are involved in the pathology of the disease, while in other cases they may be used clinically as drugs, such as IFN, granulocyte/macrophage colony-stimulating factor (GM-CSF) and erythropoietin. There is a broad range of both cell culture assays available for cytokines (Whiteside 1994; Aggarwai 1991) and commercial ELISA kits for detecting cytokines (Ledur 1995; Mire-Sluis 1995).


Hepatocyte Growth Factor Immunol Method Human Tumor Necrosis Factor Tissue Culture Infectious Dose Secrete Regulatory Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  1. 1.Novartis Ringaskiddy Ltd.Ringaskiddy, County CorkIreland

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