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Cytokine Cell Culture Assays

  • Alice Redmond
  • Jacqueline Quinn
  • Susan Molloy
Part of the Springer Lab Manual book series (SLM)

Abstract

Cytokines are defined as secreted regulatory proteins that control the survival, growth, differentiation and effector function of tissues. Cytokines encompass those families of regulators known as growth factors, colony-stimulating factors, interleukins (ILs), lymphokines, monokines and interferons (IFNs). Measuring cytokines is an important facet of understanding the complex interactions of the immune response mediated by these multifunctional, peptide, T cell products. For example cytokines regulate the activation, growth and differentiation of many cell types and have been implicated in diseases such as arthritis, allergy and leukemia (Aria 1990). In some cases, cytokines are involved in the pathology of the disease, while in other cases they may be used clinically as drugs, such as IFN, granulocyte/macrophage colony-stimulating factor (GM-CSF) and erythropoietin. There is a broad range of both cell culture assays available for cytokines (Whiteside 1994; Aggarwai 1991) and commercial ELISA kits for detecting cytokines (Ledur 1995; Mire-Sluis 1995).

Keywords

Hepatocyte Growth Factor Immunol Method Human Tumor Necrosis Factor Tissue Culture Infectious Dose Secrete Regulatory Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aggarwal BB, Gutterman JU (1987) Human cytokines. Blackwell Scientific Publications, OxfordGoogle Scholar
  2. Arai K I, Lee F, Miyajima A, Miyatake S, Arai N, Yokota T (1990) Cytokines: coordinators of immune and inflammatory response, Annu Rev Biochem 59:783–836PubMedCrossRefGoogle Scholar
  3. Borset M, Waage A, Sundan A (1996) Hepatocyte growth factor reverses the TGF-β induced growth inhibition of CC1-64 cells. J Immunol Methods 189:59–64PubMedCrossRefGoogle Scholar
  4. Cagliero E, Roth T, Taylor AW, Lorenzi M (1995) The effect of high glucose on human endothelial cell growth and gene expression are not mediated by TGFβ. J Lab Invest 73: 667–673Google Scholar
  5. Clemens MJ, Morris AG, Gearing AJH (1987) Lymphokines and interferons. A practical appraoch. IRL, OxfordGoogle Scholar
  6. Cortii A, Poiesi C, Merli S, Cassani G (1994) Tumor necrosis factor (TNF) α quantification by ELISA and Bioassay: effects of TNFα-soluble receptor (p55) complex dissociation during assay incubations. J Immunol Methods 177:191–198CrossRefGoogle Scholar
  7. Courtney VD, Mills J (1978) The use of a clongenic assay system for the analysis of IFN. Br J Cancer 37:261–269CrossRefGoogle Scholar
  8. De Jong LO, Farner NL, Widmer MB, Giri JG (1996 ) Interaction of IL-15 with shared IL-2 receptor (β and γc subunits. J Immunol 156:1339–1348.PubMedGoogle Scholar
  9. Freshney (1992) Animal cell culture: a practical approach. IRL, OxfordGoogle Scholar
  10. Garrigue-Antar P, Barbieux I , Liebeau B , Boisteau O, Gregoire C (1996) Optimisation of the CCL64 based bioassay for TGF-(3. J Immunol Methods 189:269–274Google Scholar
  11. Hamilton JA, Whitty GA, Kola I, Hertzog PJ (1996) Endogenous INFα(β suppress colony stimulating factor CSF 1 stimulated macrophage DNA synthesis and mediates inhibitory effects of lipopolysaccharide and TNF-α. J Immunol 156:2553–2557PubMedGoogle Scholar
  12. Hang L Svanborg C, Andersson G (1994) Determination of IL-6 in human urine and epithelial supernatants. Int Arch Allergy Immunol 105: 397–403PubMedCrossRefGoogle Scholar
  13. Higuchi M, Singh SM, Aggarwal BB (1995) Characterisiation of the atopoic effects of human tumor necrosis factor: development of a highly rapid and specific bioassay for human tumor necrosis factor and lymphotoxin using target cells. J Immunol Methods 178:173–181PubMedCrossRefGoogle Scholar
  14. Hill BT, Whelan RDH (1983) Modifcation of classic clonogenic assay systems. Cell Biol Int Rep 7:617–622PubMedCrossRefGoogle Scholar
  15. James P (1996) IGFBP-5 in muscle differentiation. J Cell Biol 133:681–688CrossRefGoogle Scholar
  16. Jeannin P, Delnestte Y, Seveso M, Life P, Bonnefoy J-V (1996) IL-12 synergises with IL-2 and other stimuli in inducing L-10 production by human T cells. J Immunol 156: 3159–3164PubMedGoogle Scholar
  17. Ledur A, Fitting C, David B, Hamberger C, Cavaillon J-M (1995) Variable estimates of cytokine levels produced by commercial ELISA kits results using international reference standards. J Immunol Methods 186 171–179PubMedCrossRefGoogle Scholar
  18. Levesque A, Paquet A, Page M (1995) Improved fluorescent bioassay for the detection of tumor necrosis factor activity. J Immunol Methods 178 71–76PubMedCrossRefGoogle Scholar
  19. Lieonart R, Naf D, Browning H, Weissmann C (1996) A novel Quantitative bioassay for type 1 interferon using a recombinant indicator cell line. Biotechnology 8:1263–1266Google Scholar
  20. Lu Z-Y, Zhang X-G, Gu Z-J, Yasukawa K, Amiot M, Etrillard M, Bataille R, Klein B (1994) A highly sensitive bioassay for human interleukin -11. J Immunol Methods 173:19–26PubMedCrossRefGoogle Scholar
  21. Marshall JS, Leal-Berumem I, Nielsen L, Glibetic M, Jordana M (1996) Interleukin (IL) 10 inhibits long term IL-6 production but not performed mediator release from rat peritoneal mast cells. J Clin Invest 97: 1122–1128PubMedCrossRefGoogle Scholar
  22. Meager A (1987) Biological standardisation of cytokines. Dev Bio Standard 69:199–206Google Scholar
  23. Mire-Sluis A, Gaines-Das R, Thorpe R (1995) Immunoassays for detecting cytokines: What are they really measuring? J Immunol Methods 186 157–160PubMedCrossRefGoogle Scholar
  24. Page LA, Toop MS, Thorpe R, Mire-Sluis AR(1996) An antiproliferative bioassay for interleukin 4. J Immunol Methods 189 129–135PubMedCrossRefGoogle Scholar
  25. Pauli U, Bertonin G, Duerr M, Peterhans E (1994) A bioassay for the detection of tumor necrosis factor from eight different species: evaluation of neutralisation rates of a monoclonal antibody against TNF-α. J Immunol Methods 171:263–265.PubMedCrossRefGoogle Scholar
  26. Pestka S (1986) Interferons from 1982 to 1986. Methods Enzymol 119:3–14PubMedCrossRefGoogle Scholar
  27. Randall L A, Wadhwa M, Thorpe R, Mire-Sluis AR (1994) A novel sensitive bioassay for transforming factor β. J Immunol Methods 171:263–265CrossRefGoogle Scholar
  28. Ruff MR, Gillford, GE (1981) In: Pick E (ed) Lymphokines. Academic Press, New York, p 235Google Scholar
  29. Shanan TA, Siegel PD, Sorenson WG, Kuschenr WG, Lewis DM (1994) A new sensitive bioassay for TNE J Immunol Methods 175:181–187CrossRefGoogle Scholar
  30. Taub DD, Anver M, Oppenheim JJ, Longo DL, Murphy WJ (1996) Lymphocyte recruitment by interleukin-8 (IL-8). J Clin Invest 97:1931–1941PubMedCrossRefGoogle Scholar
  31. Watari K, Mayani H, Lee F, Dragowska W, Lansdorp P M, Schrader JW (1996) Production of interleukin 1(3 by human hematopoietic progenitor cells. J Clin Invest 97:1666–1674PubMedCrossRefGoogle Scholar
  32. Whiteside L (1994) Cytokine measurements and interpretation of cytokine assays in human disease J Clin Immunol 14:327–39PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  1. 1.Novartis Ringaskiddy Ltd.Ringaskiddy, County CorkIreland

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