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Cellular Models for In Vitro Toxicity Testing

  • Flavia Zucco
  • Isabella De Angelis
  • Annalaura Stammati
Part of the Springer Lab Manual book series (SLM)

Abstract

Compared to other biomedical disciplines, cell culture systems have only recently been systematically adopted for use in toxicology (Paganuzzi Stammati et al. 1981), due, in particular, to the difficulty in extrapolating in vitro data to the in vivo situation for regulatory purposes. However, in the last decade, technological advancements using cell culture systems, together with the important results obtained in biomedical research, have impressively promoted its use in toxicology investigations. Growth factors and substrates able to stimulate cell proliferation and/or expression of specific highly specialized functions, and sophisticated analytical techniques for mechanistic studies, together with automated procedures, have played a pivotal role.

Keywords

Cellular Model Continuous Cell Line Gentian Violet Respiration Buffer Vitro Toxicity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Babich H, Borenfreund E (1990) Application of the neutral red cytotoxicity assay to in vitro toxicology. Altern Lab Anim 18: 129–144Google Scholar
  2. Balls M, Riddell R, Horner R, Clothier R (1987) The Frame approach to the development, validation and evaluation of in vitro alternative methods. In: Goldberg AM (ed) Alternative methods in toxicology, Mary Ann Liebert, New York, pp 45–58Google Scholar
  3. Balls M, Blaauboer B, Brusick D, Frazier J, Lamb D, Pemberton M, Reinhardt C, Roberfroid M, Rosenkranz H, Schmid B, Spielmann H, Stammati AL, Walum E (1990) Report and recommendations of the CAAT/ERGATT Workshop on the validation of toxicity test procedures. ATLA 18: 313–337Google Scholar
  4. Balls M, Blaauboer BJ, Fentem JH, Bruner L, Combes R, Ekwall B, Fielder RJ, Guillouzo A, Lewis RW, Lovell DP, Reinhardt CA, Repetto G, Sladowski D, Spielmann H, Zucco F (1995) Practical aspects of the validation of toxicity test procedures. ATLA 23:129–147Google Scholar
  5. Barile F A (1994) Introduction to in vitro cytotoxicology - Mechanisms and methods. CRC, Boca RatonGoogle Scholar
  6. Bondesson I, Ekwall B, Hellberg S, Romert L, Stenberg K, Walum E (1989) MEIC: a new international multicentre project to evaluate the relevance to human toxicity of in vitro cytotoxicity test. Cell Biol Toxicol 5: 331–348PubMedCrossRefGoogle Scholar
  7. Borenfreund E, Puerner JA (1984) A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR90). J Tissue Cult Methods 8: 7–9Google Scholar
  8. Bradford M (1976) A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–254PubMedCrossRefGoogle Scholar
  9. Bridges JW, Benford DJ, Hubbard SA (1983) The design and use of in vitro toxicity test. In: Turner P (ed)Animal in scientific research: an effective substitute for man? MacMillan, Basingstoke, pp 47–68Google Scholar
  10. Bruner LH (1992) Ocular irritation. In: Frazier JM (ed)In vitro toxicity testing, Marcel Dekker, New York, pp 149–189Google Scholar
  11. Cain K, Skilleter DN (1987) Preparation and use of mitochondria in toxicological research. In: Snell K, Mullock B (eds) Biochemical toxicology - a practical approach, IRL, Oxford, pp 217–254Google Scholar
  12. Carmichael J, De Graff WG, Gazdar AF, Minna JD, Mitchell JB (1987) Evaluation of tetrazolium based semiautomated colorimetric assay: assessment of chemiosensitivity testing. Cancer Res 47: 936–942PubMedGoogle Scholar
  13. Clark L C, Wolf R, Granger D, Taylor Z (1953) Continuous recording of blood oxygen tension by polarography. J Appl Physiol 6: 189–193PubMedGoogle Scholar
  14. Clothier RH (1995) The Frame cytotoxicity test (Kenacid blue). In: O’Hare S, Atter-will CK (eds) Methods in molecular biology, vol. 43. In vitro toxicity methods. Humana, Totowa, New Jersey, pp 109–118Google Scholar
  15. Cook JA, Mitchell JB (1989) Viability measurements in mammalian cell systems. Anal Biochem 179:1–7PubMedCrossRefGoogle Scholar
  16. Curren RD, Southee JA, Spielmann H, Liebsch M, Fentem JH, Balls M (1995) The role of prevalidation in the development, validation and acceptance of alternative methods. ATLA 23: 211–217Google Scholar
  17. Decker T, Lohmann-Matthes ML (1988) A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J Immunol Meth 115: 61–69CrossRefGoogle Scholar
  18. De Leo WA (1992) Cutaneous irritancy. In: Frazier JM (ed) In vitro toxicity testing. Marcel Dekker, New York, pp 191–203Google Scholar
  19. CEC (1988) Collaborative study on relationship between in vivo primary irritation and in vitro experimental models. EC Document V/E/LUX/157/88, LuxembourgGoogle Scholar
  20. CEC (1991) Collaborative study on the evaluation of alternative methods to the eye irritation test. EC Document XI/632/91-V/E/1/131/91, LuxembourgGoogle Scholar
  21. Doyle A, Griffiths JB, Newell DG (1993) Cell and tissue culture: laboratory procedure. John Wiley, New YorkGoogle Scholar
  22. Freshney RI (1994) Culture of animal cells. A manual of basic technique, 3rd edn. Wiley-Liss, New YorkGoogle Scholar
  23. Frazier JM (1992) In vitro toxicity testing - application to safety evaluation. Marcel Dekker, New YorkGoogle Scholar
  24. Gad SC (1994) In vitro toxicology. Raven, New YorkGoogle Scholar
  25. Gad SC (1993) Alternatives to in vivo studies in toxicology. In: Ballantyne B, Marrs T, Turner P (eds) General and applied toxicology, vol. I. Stockon, New York, pp 179–206Google Scholar
  26. Garle MJ, Fentem JH, Fry JR (1994) In vitro cytotoxicity tests for the prediction of acute toxicity in vivo. Toxicol In Vitro 8: 1303–1312PubMedCrossRefGoogle Scholar
  27. Goldberg AM (1983-1995) Alternative methods in toxicology, vol. 1–11. Mary Ann Liebert, New YorkGoogle Scholar
  28. Gordon WC, Harvell J, Bason M, Maibach H (1994) In vitro methods to predict dermal toxicity. In: Gad SC (ed) In vitro toxicology, Raven, New York, pp 47–55Google Scholar
  29. Green S, Chambers WA, Gupta KC, Hill RN, Hurley PM, Lambert LA, Lee CC, Lee JK, Liu PT, Lowther DK, Roberts CD, Seabaugh WM, Spronger JA, Wilcox NL (1993) Criteria for in vitro alternatives for the eye irritation test. Food Chem Toxicol 31: 81–85PubMedCrossRefGoogle Scholar
  30. Herzinger T, Korting HT, Maibach HI (1995) Assessment of cutaneous and ocular irritancy: a decade of research on alternatives to animal experimentation. Fund Appl Toxicol 24: 29–41CrossRefGoogle Scholar
  31. Hornhardt S, Wiebel FJ (1996) Catalogue of cell lines in toxicology and pharmacology. GFS-Forschungszentrum für umwelt und gesundheit, Bericht 3/96. ISSN 0721–1694 Jolles G, Cordier A (1992) In vitro methods in toxicology. Rhone-Poulenc Rorer Foundation, Academic Press, New YorkGoogle Scholar
  32. Knox P, Uphill PF, Fry JR, Benford J, Balls M (1986) The Frame multicentre project on in vitro cytotoxicology. Food Chem Toxicol 24: 457–463PubMedCrossRefGoogle Scholar
  33. Loprieno N (1995) Alternative methodologies for the safety evaluation of chemicals in the cosmetic industry. CRC, Boca RatonGoogle Scholar
  34. Lowry OH, Rosenbrough NJ, Farr AL, Randall R L (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275PubMedGoogle Scholar
  35. Martin A, Clynes M (1993) Comparison of 5 microplate colorimetric assays for in vitro cytotoxicity testing and cell proliferation assays. Cytotechnology 11: 49–58PubMedCrossRefGoogle Scholar
  36. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Meth 65: 55–63CrossRefGoogle Scholar
  37. Nardone RM (1977) Toxicity testing in vitro. In: Rothblat GH, Cristofalo VJ (eds) Growth, nutrition and metabolism of cells in culture. Academic Press, New York, pp 471–491Google Scholar
  38. O' Hare S, Atterwill CK (1995) Methods in molecular biology, vol. 43: In vitro toxicity testing protocols. Humana, Totowa, New JerseyCrossRefGoogle Scholar
  39. Paganuzzi Stammati A, Silano V, Zucco F (1981) Toxicology investigations with cell culture systems. Toxicology 20: 91–153CrossRefGoogle Scholar
  40. Rodrigues AD (1994) Use of in vitro human metabolism studies in drug development. An industrial perspective. Biochem Pharmacol 48: 2147–2156Google Scholar
  41. Rogiers V, Sonck W, Shephard E, Vercruysse A (1993) Human cells in in vitro pharmacotoxicology. VUB, BrusselsGoogle Scholar
  42. Schnellmann RG (1994) Measurement of oxygen consumption. In: Tyson CA, Frazier JM (eds) Methods in toxicology, part B: in vitro toxicity indicators, Academic Press, New York, pp 129–139Google Scholar
  43. Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Visitica D T, Warren J, Bokesch H, Kenney S, Boyd MR (1990) New colorimetric assay of anticancer drug screening. J Nat Cancer Inst 82: 1107–1113PubMedCrossRefGoogle Scholar
  44. Skehan P (1995) Assay of cell growth and cytotoxicity. In: Studzinski GP (ed) Cell growth and apoptosis - a practical approach series, IRL, Oxford, pp 169–191Google Scholar
  45. Sina JF, Gautheron PD (1994) Ocular toxicity assessment in vitro, In: Gad SC (ed) In vitro toxicology. Raven, New York, pp 21–45Google Scholar
  46. Skett P, Tyson C, Guillouzo A, Maier P (1995) Report on the international workshop on the use of human in vitro liver preparations to study drug metabolism in drug development. Biochem Pharmacol 50: 280–285PubMedCrossRefGoogle Scholar
  47. Stark DM, Shopsis C, Borenfreund E, Babich H (1986) Progress and problems in evaluating and validating alternative assays in toxicology. Food Chem Toxicol 24: 449–455PubMedCrossRefGoogle Scholar
  48. Supino R (1995) MTT assay. In: O’Hare S and Atterwill CK (eds) Methods in molecular biology, vol 43. In vitro toxicity testing protocols. Humana, Totowa, New Jersey, pp 137–149Google Scholar
  49. Tyson CA, Frazier JM (1994) Methods in toxicology, vols. 1A, 1B. Academic Press, San DiegoGoogle Scholar
  50. Vassault A (1983) Lactate dehydrogenase: UV method with piruvate and NADH. In: Bergmeyer HU, Bergmeyer J, Grassl M (eds) Methods of enzymatic analysis. III. Enzymes 1: oxidoreductases, transferases, 3rd edn. Verlag-Chemie, Weinheim, pp 118–126Google Scholar
  51. Watson RR (1992) In vitro methods of toxicology. CRC, Boca RatonGoogle Scholar
  52. Welder AA, Acosta D (1994) Enzyme leakage as an indicator of cytotoxicity in cultured cells. In: Tyson CA, Frazier JM (eds) Methods in toxicology, part B. In vitro toxicity indicators. Academic Press, New York, pp 46–49Google Scholar
  53. Wilson AP (1992) Cytotoxicity and viability assay. In: Freshney RI (ed) Animal cell cultures - a practical approach series, 2nd edn. IRL, Oxford, pp 263–303Google Scholar
  54. Wrighton SA, Vandenbranden M, Stevens JC et al.: Shipley LA, Ring BJ, Rettie AE, Cashman JR (1993) In vitro methods for assessing human hepatic drug metabolism: their use in drug development. Drug Met Rev 25: 453–484CrossRefGoogle Scholar
  55. Zucco F (1992) Use of continuous cell lines for toxicological studies. In: Castell JV, Gomez-Léchon M (eds) In vitro alternatives to animal pharmaco-toxicology. Farmindustria, Serie Cientifka, Madrid, pp 43–68Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Flavia Zucco
    • 1
  • Isabella De Angelis
    • 2
  • Annalaura Stammati
    • 2
  1. 1.Istituto Tecnologie BiomedicheC.N.RRomeItaly
  2. 2.Laboratorio di Tossicologia Comparata ed EcotossicologiaIstituto Superiore di SanitàRomeItaly

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