Abstract
Myeloid leukemic cell lines established from patients with either acute myeloid leukemia (AML) or the blastic phase of chronic myeloid leukemia (CML) are arrested at different stages of myeloid development. These lines have been widely studied as models of myeloid differentiation and as a substitute for their more mature, normal myeloid counterparts which sometimes are cumbersome to use. The most broadly studied human myeloid cell line, the human acute myeloid leukemia HL-60 cell line, was derived from a 36-year-old woman diagnosed and treated at M.D. Anderson Hospital in Texas (Collins et al. 1977). This patient was initially considered to have acute promyelocytic leukemia (APL, also named French-American-British classification M3 or FAB-M3), but had atypical clinical features, indicating that the leukemia from which the HL-60 cells were derived was more appropriately classified as an AML with maturation, FAB-M2 (Dalton et al. 1988). APL represents a model for the therapeutic approach of differentiation therapy, as APL cells are able to respond to all-trans retinoic acid (RA) treatment by terminal differentiation (Huang et al. 1988). The chromosomal translocation t(15;17) in the blasts is specifically observed in APL, leading to a chimeric gene between the COOH-terminal of the RA receptor (RARa) and the NH2-terminal of a myeloid gene product initially referred to as Myl (de Thé et al. 1990) and then renamed PML (promyelocytic leukemia).
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© 1998 Springer-Verlag Berlin Heidelberg
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Mollinedo, F., Santos-Beneit, A.M., Gajate, C. (1998). The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses. In: Clynes, M. (eds) Animal Cell Culture Techniques. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80412-0_16
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DOI: https://doi.org/10.1007/978-3-642-80412-0_16
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