Abstract
Mononuclear phagocytes are ubiquitous cells and practically all tissues can be used as sources for obtaining them, most commonly: bone marrow, thymus, spleen, blood, peritoneal cavity, lungs, brain, skin. In this chapter, particular attention will be focused on the primary culture models developed from murine peritoneal macrophages and human peripheral blood monocytes; the use of these systems is recommended by the Society for Leukocyte Biology (Morahan 1980). The description of methods for obtaining other types of mononuclear phagocytes or f studying them can be found in specialized handbooks (Adams 1979 Adams et al. 1981; Beelen and Poindron 1994).
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© 1998 Springer-Verlag Berlin Heidelberg
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Poindron, P., Lombard, Y. (1998). Cell Culture Models for Mononuclear Phagocytes. In: Clynes, M. (eds) Animal Cell Culture Techniques. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80412-0_15
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DOI: https://doi.org/10.1007/978-3-642-80412-0_15
Publisher Name: Springer, Berlin, Heidelberg
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