Skip to main content
SpringerLink
Log in

The Granulocyte/Macrophage Colony-Forming Unit Assay

  • Chapter
Book cover Animal Cell Culture Techniques

Part of the book series: Springer Lab Manual ((SLM))

Abstract

Although the complex dynamics of the interactions among the different components of the hematopoietic system remains poorly understood, much progress has been made in identifying the various cell types and the multitude of growth factors regulating its homeostasis. For example, the existence of pluripotent stem cells (with an infinite capacity of self-renewal), progenitor cells (with a finite capacity for self-renewal), committed stem cells (oriented toward a specific lineage differentiation) and mature cells (fully differentiated that do not divide) has been established (Fig. 13.1).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 119.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bradley TR, Metcalf D (1966) The growth of mouse bone marrow cells in vitro. Austr J Exp Biol Med 44: 287–289

    Article  CAS  Google Scholar 

  • Deldar A, House RV, Wierda D (1995) Bone marrow colony-forming assays. Methods Immunotoxicol 1:227–250

    CAS  Google Scholar 

  • Dexter TM (1979) Hemopoiesis in long-term bone marrow culture. Acta Hemat 62: 299–304

    Article  CAS  Google Scholar 

  • Dexter TM, Allen TD, Lajtha LG (1977) Conditions controlling the proliferation of hematopoietic cells in vitro. J Cell Physiol 91: 335–344

    Article  PubMed  CAS  Google Scholar 

  • Greenberger JS (1991) The hematopoietic microenviroment. Crit Rev Oncol Hematol 11:65–84

    Article  PubMed  CAS  Google Scholar 

  • Gribaldo L,Bueren J, Deldar A, Hokland P, Meredith C, Moneta P, Mosesso R, Parchment R, Parent-Massin D, Pessina P, San Roman J, Scoeters G (1996) Alternatives to Laboratory Animals 24:211–231

    Google Scholar 

  • Guigon M, Bonnet O (1995) Inhibitory peptides in hematopoiesis. Exp Hematol 23: 477–481

    PubMed  CAS  Google Scholar 

  • Klein G (1995) The extracellular matrix of the hematopoietic microenviroment. Experientia 51: 914–926

    Article  PubMed  CAS  Google Scholar 

  • Konshansky K, Karplus PA (1993) Hematopoietic growth factors: understanding functional diversity in structural terms. Blood 82: 3229–3240

    Google Scholar 

  • McNiece IK, Langley KE, Zsebo KM (1991) The role of recombinant stem cell factor in early B cell development. J Immunol 146: 3785–3790

    PubMed  CAS  Google Scholar 

  • Metcalf D (1980) Clonal analysis of proliferation and differentiation of paired daughter cells: Action of granulocyte-macrophage colony stimulating factors on granulomacrophage precursors. Proc Natl Acad Sci USA 77:5327–5330

    Article  PubMed  CAS  Google Scholar 

  • Metcalf D (1989) The molecular control of cell division, differentiation commitment and maturation in hematopoietic cells. Nature 274:168–169

    Google Scholar 

  • Nakahata T, Gross AJ, Ogawa M (1982) A stochastic model of self-renewal and commitment to differentiation of the primitive hematopoietic stem cells in culture. J Cell Physiol 113:455–458

    Article  PubMed  CAS  Google Scholar 

  • Naughton BA, Naughton GK (1989) Hematopoiesis on nylon mesh templates. Annals NY Acad Sci 554:125–140

    Article  CAS  Google Scholar 

  • Nishino T, Hisha H, Nishino N, Adach M, Ikehara S (1995) Hepatocyte growth factors as a hematopoietic regulator. Blood 85: 3093–3100

    PubMed  CAS  Google Scholar 

  • Ogawa M (1993) Differentiation and proliferation of hematopoietic stem cells. Blood 81:2844–2853

    PubMed  CAS  Google Scholar 

  • Parchment RE, Huang M, Erickson-Miller CL (1993) Roles for in vitro myelotoxicity tests in preclinical drug development and clinical trial planning. Toxicol Pathol 21:241–250

    Article  PubMed  CAS  Google Scholar 

  • Parker JW, Metcalf D (1974) Production of colony stimulating factor in mitogen stimulated lymphocyte cultures. J Immunol 112:502–510

    PubMed  CAS  Google Scholar 

  • Pessina A, Neri MG, Muschiato A, Brambilla P, Marocchi A, Mocarelli P (1986) Colony stimulating factor produced by murine adrenocortical tumor cells. J Natl Cancer Inst 76:1095–1099

    PubMed  CAS  Google Scholar 

  • Pessina A, Mineo E, Neri MG, Gribaldo L, Colombo R, Brambilla P, Zaleskis G (1992) Establishement and characterization of a new murine cell line(SR-4987) derived from marrow stromal cells. Cytotechnology 8:93–102

    Article  PubMed  CAS  Google Scholar 

  • Pluznick DH, Sachs L (1965) The cloning of normal “mast cells” in tissue culture. J Cell Physiol 66: 319–325

    Article  Google Scholar 

  • Suda T, Suda J, Ogawa M (1983) Single cell origin of mouse hematopoietic colonies expressing multiple lineages in variable combinations. Proc Natl Acad Sci USA 80: 6689–6693

    Article  PubMed  CAS  Google Scholar 

  • Till JE, Mc Culloch EA (1961) A direct measurament of the radiation sensitivity of normal mouse bone marrow cells. Radiation Res 14: 213–222

    Article  PubMed  CAS  Google Scholar 

  • Vogel H, Niewish H, Matioli G (1969) Stochastic development of stem cells. J Theoret Biol 22: 249–270

    Article  CAS  Google Scholar 

  • Wang QR, Wolf NS (1990) Dissecting of hematopoietic microenviroment with clonal isolation and identification of cell types in murine CFU-F colonies by limiting dilution. Exp Hematol 18: 355–361

    PubMed  CAS  Google Scholar 

  • Watt SM, Davis JM (1989) Flow sorting for isolating CFU-E. In: Pollard JW and Walker JM (eds) Methods in molecular biology, vol.V. Humana, Clifton, New Jersey, pp 347–360

    Google Scholar 

  • Weiss MJ, Orkin SH (1995) Gata transcription factors: key regulators of hematopoiesis. Exp Hematol 23: 99–107

    PubMed  CAS  Google Scholar 

  • Whitlock CA, Witte ON (1982) Long term culture of B lymphocytes and their precursor from murine bone marrow. Proc Natl Acad Sci USA 79: 3608–3612

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Cell Culture, Institute of Microbiology, Faculty of Medicine, University of Milan, 36 Via Pascal, Italy

    Augusto Pessina

Authors
  1. Augusto Pessina
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Augusto Pessina .

Editor information

Editors and Affiliations

  1. National Cell & Tissue Culture Center Bioresearch Ireland, Dublin City University, Glasnevin Dublin 9, Ireland

    Martin Clynes

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Pessina, A. (1998). The Granulocyte/Macrophage Colony-Forming Unit Assay. In: Clynes, M. (eds) Animal Cell Culture Techniques. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80412-0_13

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-80412-0_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-63008-1

  • Online ISBN: 978-3-642-80412-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation