Cotton pp 107-120 | Cite as

In Vitro Culture of Cotton Ovules

  • J. E. Mellon
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 42)

Abstract

In vitro culture of developing cotton ovules is a significant achievement in the continuing investigation of cotton and its agronomic properties. Pioneering work on in vitro growth of cotton ovules met with limited sucess, as ovule growth was abnormal and fiber development did not occur (Joshi 1960). Later, the effects of hormones, casein hydrolysate, and yeast extract on the development of the cotton embryo and integuments were reported (Joshi and Johri 1972). Beasley and coworkers began development of the cotton ovule culture system in 1971 (see Beasley 1977) in order to conduct investigations on fiber development. Eid et al. (1973) were successful in culturing 10-day postanthesis ovules on MS medium (Murashige and Skoog 1962), but the resulting embryos did not develop normally. Beasley and Ting (1973) subsequently developed a phytohormone-supplemented medium (BT) derived from the MS medium which supported the development of the ovules in a manner close to that observed in vivo. The BT medium could be utilized for both fertilized (Beasley and Ting 1973) and unfertilized (Beasley and Ting 1974) ovules. While this medium supported normal growth of the ovule and fiber during the first 2 weeks of development, the embryos did not continue to develop normally, and subsequently degenerated. Stewart and Hsu (1977) resolved this problem by inclusion of an ammonium ion amendment to the medium; by making this medium modification, 50% of the ovules produced physiologically mature embryos.

Keywords

Cellulose Boron Respiration Polysaccharide Methionine 

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References

  1. Altman DW (1988) Exogenous hormone applications at pollination for in vitro and in vivo production of cotton interspecific hybrids. Plant Cell Rep 7:257–261CrossRefGoogle Scholar
  2. Altman DW, Stelly DM, Kohel RJ (1987) Introgression of the glanded-plant and glandless-seed trait from Gossypium sturtianum Willis into cultivated upland cotton using ovule culture. Crop Sci 27:880–884CrossRefGoogle Scholar
  3. Bajaj YPS, Gill MS (1985) In vitro induction of genetic variability in cotton (Gossypium spp.). Theor Appl Genet 70:363–368Google Scholar
  4. Bajaj YPS, Gill MS (1992) Micropropagation of cotton (Gossypium species). In: Bajaj YPS (ed) High-tech and micropropagation III. Biotechnology in agriculture and forestry, vol 19. Springer, Berlin Heidelberg New York, pp 483–504Google Scholar
  5. Beasley CA (1977) Ovule culture: fundamental and pragmatic research for the cotton industry. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 160–178Google Scholar
  6. Beasley CA (1984) Culture of cotton ovules. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 1. Academic Press, New York, pp 232–240Google Scholar
  7. Beasley CA (1992) In vitro cotton ovule culture: a review. In: Proc Cotton Fiber Cellulose: Structure, Function and Utiliztion. National Cotton Council of America, Memphis, TN, pp 65–89Google Scholar
  8. Beasley CA, Egli E (1977) Fiber production in vitro from a conditional fiberless mutant of cotton. Dev Biol 57:234–237PubMedCrossRefGoogle Scholar
  9. Beasley CA, Ting IP (1973) The effects of plant growth substances on in vitro fiber development from fertilized cotton ovules. Am J Bot 60:130–139CrossRefGoogle Scholar
  10. Beasley CA, Ting IP (1974) Effects of plant growth substances on in vitro fiber development from unfertilized cotton ovules. Am J Bot 61:188–194CrossRefGoogle Scholar
  11. Birnbaum EH, Dugger WM, Beasley CA (1977) Interaction of boron with components of nucleic acid metabolism in cotton ovules cultured in vitro. Plant Physiol 59:1034–1038PubMedCrossRefGoogle Scholar
  12. Buchala AJ, Roulin S, Meier H (1989) Polysaccharides in the culture medium of cotton (Gossypium hirsutum L.) ovules cultured in vitro. Plant Cell Rep 8:25–28CrossRefGoogle Scholar
  13. Carpita NC, Delmer DP (1981) Concentration and metabolic turnover of udp-glucose in developing cotton fibers. J Biol Chem 256:308–315PubMedGoogle Scholar
  14. Contolini CS, Menzel MY (1987) Early development of duplication-deficiency ovules in upland cotton. Crop Sci 27:345–348CrossRefGoogle Scholar
  15. Davidonis GH (1989) Fiber development in preanthesis cotton ovules. Physiol Plant 75:290–294CrossRefGoogle Scholar
  16. Davidonis GH (1993a) Cotton fiber growth and development in vitro: effects of tunicamycin and monensin. Plant Sci 88:229–236CrossRefGoogle Scholar
  17. Davidonis GH (1993b) A comparison of cotton ovule and cotton cell suspension cultures: response to gibberellic acid and 2-chloroethylphosphonic acid. J Plant Physiol 141:505–507Google Scholar
  18. Davidonis GH, Hinojosa O (1994) Influence of seed location on cotton fiber development in planta and in vitro. Plant Sci 103: 107–113CrossRefGoogle Scholar
  19. Dhindsa RS (1978a) Hormonal regulation of cotton ovule and fiber growth: effects of brom- odeoxyuridine, amo-1618 and p-chlorophenoxyisobutyric acid. Planta 141:269–272CrossRefGoogle Scholar
  20. Dhindsa RS (1978b) Hormonal regulation of enzymes of nonautotrophic CO2 fixation in unfertilized ovules. Z Pflanzenphysiol 89:355–362Google Scholar
  21. Dugger WM, Palmer RL (1980) Effect of boron on the incorporation of glucose from udp-glucose into cotton fibers grown in vitro. Plant Physiol 65:266–273PubMedCrossRefGoogle Scholar
  22. Dugger WM, Palmer RL (1985) Effect of boron on the incorporation of glucose by cotton fibers grown in vitro. J Plant Nutr 8:311–325CrossRefGoogle Scholar
  23. Eid AAH, DeLange E, Waterkeyn L (1973) In vitro culture of fertilized cotton ovules. I. The growth of cotton embryos. Cellule 69:361–371Google Scholar
  24. Francey Y, Jaquet JP, Cairoli S, Buchala AJ, Meier H (1989) The biosynthesis of β-glucans in cotton (Gossypium hirsutum L.) fibres of ovules cultured in vitro. J Plant Physiol 134:485–491Google Scholar
  25. Gill MS, Bajaj YPS (1987) Hybridization between diploid (Gossypium arboreum) and tetraploid (Gossypium hirsutum) cotton through ovule culture. Euphytica 36:625–630CrossRefGoogle Scholar
  26. Gould JH, Palmer RL, Dugger WM (1986) Isolation and culture of cotton ovule epidermal protoplasts (prefiber cells) and analysis of the regenerated wall. Plant Cell Tissue Organ Cult 6:47–59CrossRefGoogle Scholar
  27. Graves DA, Stewart JM (1988) Chronology of the differentiation of cotton (Gossypium hirsutum L.) fiber cells. Planta 175:254–258CrossRefGoogle Scholar
  28. Haigler CH, Rao NR, Roberts EM, Huang JY, Upchurch DR, Trolinder NL (1991) Cultured ovules as models for cotton fiber development under low temperatures. Plant Physiol 95:88–96PubMedCrossRefGoogle Scholar
  29. Joshi PC (1960) In vitro growth of cotton ovules. Symp Plant Embryology. Council of Scientific and Industrial Res, New Delhi, pp 199–204Google Scholar
  30. Joshi PC, Johri BM (1972) In vitro growth of ovules of Gossypium hirsutum. Phytomorphology 22:195–209Google Scholar
  31. Kaur K, Nayyar H, Basra AS, Malik CP (1990) Stimulation of enzymes of non-photosynthetic C4 metabolism in cultured cotton ovules by fluridone. Acta Physiol Plant 12:3–6Google Scholar
  32. Meinert MC, Delmer DP (1977) Changes in biochemical composition of the cell wall of the cotton fiber during development. Plant Physiol 59:1088–1097PubMedCrossRefGoogle Scholar
  33. Mellon JE (1986) Some characteristics of peroxidase secreted by cotton ovule cultures. Plant Cell Rep 5:338–341CrossRefGoogle Scholar
  34. Mellon JE (1988) Use of ovule culture as a model for cotton-Aspergillus flavus interactions. In: Brown JM (ed) Proc Beltwide Cotton Prod Res Conf, National Cotton Council of America, Memphis, TN, pp 35–37Google Scholar
  35. Mellon JE (1991) Purification and characterization of isoperoxidases elicited by Aspergillus flavus in cotton ovule cultures. Plant Physiol 95:14–20PubMedCrossRefGoogle Scholar
  36. Mellon JE (1992) Inhibition of aflatoxin production in Aspergillus flavus by cotton ovule extracts. J Am Oil Chem Soc 69:945–947CrossRefGoogle Scholar
  37. Mellon JE, Triplett BA (1989) De novo synthesis of peroxidase in cotton ovule culture medium. Physiol Plant 77:302–307CrossRefGoogle Scholar
  38. Menzel MY, Hasenkampf CA, Stewart JM (1982) Incipient genome differentiation in Gossypium. III. Comparison of chromosomes of Gossypium hirsutum and Asiatic diploids using heterozygous translocations. Genetics 100:89–103PubMedGoogle Scholar
  39. Montezinos D, Delmer DP (1980) Characterization of inhibitors of cellulose synthesis in cotton fibers. Planta 148:305–311CrossRefGoogle Scholar
  40. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  41. Nayyar H, Kaur K, Basra AS, Malik CP (1989) Hormonal regulation of cotton fibre elongation in Gossypium arboreum L. in vitro and in vivo. Biochem Physiol Pflanz 185:415–421Google Scholar
  42. Ramsey P, Menke S, Zeiher C (1996) Stimulation of PEP carboxylase by auxin and gibberellin in cultured cotton ovules. Plant Physiol 111(S):114Google Scholar
  43. Roberts EM, Rao NR, Huang JY, Trolinder NL, Haigler CH (1992) Effects of cycling temperatures on fiber metabolism in cultured cotton ovules. Plant Physiol 100:979–986PubMedCrossRefGoogle Scholar
  44. Ryser U, Meier H, Holloway PJ (1983) Identification and localization of suberin in the cell walls of green cotton fibres (Gossypium hirsutum L., var. Green Lint). Protoplasma 117:196–205CrossRefGoogle Scholar
  45. Schmutz A, Jenny T, Amrhein N, Ryser U (1993) Caffeic acid and glycerol are constituents of the suberin layers in green cotton fibres. Planta 189:453–460CrossRefGoogle Scholar
  46. Seagull RW (1986) Changes in microtubule organization and microfibril orientation during in vitro cotton fiber development: an immunofluorescent study. Can J Bot 64:1373–1381CrossRefGoogle Scholar
  47. Seagull RW (1989) Changes in microtubule arrays during cotton fiber development. In: Bailey GW (ed) Proc 47th Annu Meet Electr Micros Soc Am, San Francisco Press, San Francisco, pp 760–761Google Scholar
  48. Seagull RW (1990) The effects of microtubule and microfilament disrupting agents on cytoskeletal arrays and wall depositon in developing cotton fibers. Protoplasma 159:44–59CrossRefGoogle Scholar
  49. Seagull RW (1992) A quantitative electron microscopic study of changes in microtubule arrays and wall microfibril orientation during in vitro cotton fiber development. J Cell Sci 101:561–577Google Scholar
  50. Stewart JM (1981) In vitro fertilization and embryo rescue. Environ Exp Bot 21:301–315CrossRefGoogle Scholar
  51. Stewart JM (1991) Biotechnology of cotton. CAB International, Wallingford, pp 21–22Google Scholar
  52. Stewart JM, Hsu CL (1977) In-ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.). Planta 137:113–117CrossRefGoogle Scholar
  53. Stewart JM, Hsu CL (1978) Hybridization of diploid and tetraploid cottons through in-ovulo embryo culture. J Hered 69:404–408Google Scholar
  54. Thengane S, Paranjpe SV, Khuspe SS, Mascarenhas AF (1986) Hybridization of Gossypium species through in-ovulo embryo culture. Plant Cell Tissue Organ Cult 6: 209–219CrossRefGoogle Scholar
  55. Triplett BA, Timpa JD (1995) Characterization of cell-wall polymers from cotton ovule culture fiber cells by gel permeation chromatography. In Vitro Cell Dev Biol 31:171–175Google Scholar
  56. Triplett BA, Busch WH, Goynes WR Jr (1989) Ovule and suspension culture of a cotton fiber development mutant. In Vitro Cell Dev Biol 25:197–200CrossRefGoogle Scholar
  57. Trolinder NL, Berlin JD, Goodin JR (1987) Differentiation of cotton fibers from single cells in suspension culture. In Vitro Cell Dev Biol 23:789–794CrossRefGoogle Scholar
  58. Wainwright IM, Palmer RL, Dugger WM (1980) Pyrimidine pathway in boron-deficient cotton fiber. Plant Physiol 65:893–896PubMedCrossRefGoogle Scholar
  59. Xie W, Trolinder NL, Haigier CH (1993) Cool temperature effects on cotton fiber initiation and elongation clarified using in vitro cultures. Crop Sci 33:1258–1264CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • J. E. Mellon
    • 1
  1. 1.USDA/ARS, Southern Regional Research CenterNew OrleansUSA

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