Abstract
For interspecific hybridization in various crops, in addition to sexual hybridization through embryo rescue and somatic hybridization by protoplast fusion, intraovarian pollination and in vitro fertilization techniques can be used to produce hybrids in incompatible crosses. The technique of in vitro pollination/ fertilization involves growing unpollinated ovaries/flowers/ovules on synthetic media, and then sprinkling pollen over them, or onto the cut end of the style. This technique has been used for seed production in a number of plant species (see Bajaj 1979; Rafaat et al. 1984; Zenkteler 1990; Kranz and Loerz 1993).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bajaj YPS (1966) Growth of Hyoscyamus niger ovaries in culture. Phyton 23:57–62
Bajaj YPS (1979) Test-tube fertilization and development of maize (Zea mays L.) plants. Indian J Exp Biol 17:475–478
Bajaj YPS (1990) Wide hybridization in legumes and oilseed crops through embryo, ovule, and ovary culture. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 10. Legumes and oilseed crops I. Springer, Berlin Heidelberg New York, pp 3–37
Bajaj YPS, Gill MS (1985) In vitro induction of genetic variability in cotton (Gossypium spp.). Theor Appl Genet 70:363–368
Beasley CA, Ting IP (1973) The effects of plant growth substances on in vitro fibre development from fertilized cotton ovules. Am J Bot 60:130–139
Gill MS, Bajaj YPS (1987) Hybridization between diploid (Gossypium arboreum) and tetraploid (Gossypium hirsutum) cotton through ovule culture. Euphytica 36:625–630
Kranz E, Loerz H (1993) In vitro fertilization with isolated, single gametes results in zygotic embryogenesis and fertile maize plants. Plant Cell 5:739–746
Liu Chengzhi, Shun J, Liu J (1992) In vitro interspecific fertilization, embryo development and formation of hybrid seedlings between Gossypium hirsutum and G. arboreum. Euphytica 60:79–88
Pundir NS (1972) Experimental embryology of Gossypium arboreum and G. hirsutum and their reciprocal crosses. Bot Gaz 133:7–26
Refaat M, Rossignol L, Demarly Y (1984) Interspecific hybrid of Gossypium hirsutum L. X G. barbadense L. via in vitro fertilization and ovule culture. Z Pfanzenzücht 93:137–146
Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204
Taylor RM (1972) Germination of cotton pollens on an artificial medium. Crop Sci 12:243–244
Weaver JB Jr (1958) Embryological studies following interspecific crosses in Gossypium II. G. arboreum X G. hirsutum. Am J Bot 45:10–16
Williams EG, Bruce Knox R, Rouse JL (1982) Pollination subsystems distinguished by pollen tube arrest after incompatible interspecific crosses in Rhododendron (Ericaceae). J Cell Sci 53:255–277
Zenkteler M (1990) In vitro fertlization and wide hybridization in higher plants. Crit Rev Plant Sci 9:267–279
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Liu, C., Bajaj, Y.P.S. (1998). In Vitro Fertilization in Cotton. In: Bajaj, Y.P.S. (eds) Cotton. Biotechnology in Agriculture and Forestry, vol 42. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80373-4_6
Download citation
DOI: https://doi.org/10.1007/978-3-642-80373-4_6
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-80375-8
Online ISBN: 978-3-642-80373-4
eBook Packages: Springer Book Archive