Genetic Transformation of Cotton Through Particle Bombardment
Plant transformation is a process by which an identifiable DNA segment is inserted into a plant genome. The introduced DNA is integrated into the chromosome, which may or may not be transcriptionally active, and is transmitted to the sexual offspring. Thus, transformation enables us to modify the genetic makeup of a plant, alter gene expression, and enhance agronomical or product traits. Although plant protoplasts allow free access to the DNA, the cells that have taken up the DNA have to be grown into a whole plant. However, since not all cells are totipotent, transformation methodologies that depend on regeneration of protoplasts through tissue culture are limited to certain cultivars. To overcome this shortcoming, direct DNA introduction into growing meristems has been developed.
KeywordsTransformation Frequency Particle Bombardment Gossypium Hirsutum Transgenic Cotton Pink Bollworm
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