Skip to main content
SpringerLink
Log in

Viscoelasticity, Rheology and Molecular Conformational Dynamics of Entangled and Cross-Linked Actin Networks

  • Chapter
Modern Optics, Electronics and High Precision Techniques in Cell Biology

Part of the book series: Principles and Practice ((PRINCIPLES))

  • 399 Accesses

Abstract

This chapter deals with the viscoelastic properties of purely entangled and cross-linked networks of actin. It concentrates on experimental techniques, physical concepts and biological implications. In particular the question of correlations between the phenomenological viscoelastic parameters (measured for instance in a rheometer) and the conformational dynamics or the bending elasticity of single polymer filaments is addressed. Another central aim is to show that high precision measurements of viscoelastic properties provide a very useful tool to detect and analyse (even subtle) effects of the manifold of actin manipulation proteins on the structural and dynamic properties of the actin-based cytoskeleton.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bastide J, Leibler L, Prost J (1990) Enhancement of inhomogeneities in gels upon swelling and stretching. Makromolecules 23:1821–1828

    Article  CAS  Google Scholar 

  • Colby RH, Gillmor JR, Rubinstein M (1993) Dynamics of near critical polymer gels. Phys Rev E 48:3712–3715

    Article  CAS  Google Scholar 

  • deGennes P (1979) Scaling concepts in polymer physics. Cornell University Press, Ithaca

    Google Scholar 

  • Doi M, Edwards SF (1986) The theory of polymer dynamics. Clarendon Press, Oxford

    Google Scholar 

  • Evans EA (1993) New physical concepts for cell amoeboid motion. Biophys J 64:1306–1322

    Article  PubMed  CAS  Google Scholar 

  • Ferry JD (1980) Viscoelastic properties of polymers. Wiley, London

    Google Scholar 

  • Forgacs G (1995) On the possible role of cytoskeletal filamentous networks in intracellular signaling:an approach based on percolation. J Cell Sci 108:2131–2143

    PubMed  CAS  Google Scholar 

  • Fuller GG, Mikkelsen K (1988) Optical rheometry using a rotatory polarization modulation. J Rheol 33:701–769

    Google Scholar 

  • Fung YC (1981) Biomechanics:Mechanical properties of living tissue. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Götter R, Kroy K, Frey E, Bärmann M, Sackmann E (1996) Dynamic light scattering from semidilute actin solutions:a study of hydrodynamic screening, filament bending stiffness and the effect of tropomyosin/troponin binding. Macromolecules 29:30–36

    Article  Google Scholar 

  • Hinner B (1997) Messung viskoelastische Eigenschaften halbvedünnter und konzentrierter Aktinlösungen. Diploma Thesis, Technical University Munich

    Google Scholar 

  • Isambert H, Maggs AC (1996) Dynamics and rheology of actin solutions. Macromolecules 29:1036–1040

    Article  CAS  Google Scholar 

  • Isenberg G (1991) Actin binding proteins — lipid interactions. J Muscle Res Cell Motility 12:136–144

    Article  CAS  Google Scholar 

  • Janmey P (1995) Cell membranes and cytoskeleton. In:Lipowsky P, Sackmann E (eds) Structure and dynamics of membranes. Handbook of biological physics, vol 1B. Elsevier — North Holland, Amsterdam

    Google Scholar 

  • Janmey PA, Hvit S, Lamb J, Stossel TP (1990) Resemblance of actin-binding protein/actin gels to covalently cross-linked networks. Nature 345:89–92

    Article  PubMed  CAS  Google Scholar 

  • Janmey P, Käs J, Sackmann E et al. (1994) The mechanical properties of actin gels elastic modulus and filament motions. J Biol Chem 269:32503–32513

    PubMed  CAS  Google Scholar 

  • Kaufmann S, Käs J, Goldmann WH, Sackmann E, Isenberg G (1992) Talin anchors and nucleates actin filaments at lipid membranes:a direct demonstration. FEBS Lett 314:203–205

    Article  PubMed  CAS  Google Scholar 

  • Kavassalis TA, Noolandi J (1988) A new theory of entanglements and dynamics in dense polymer systems. Macromolecules 21:2869–2879

    Article  CAS  Google Scholar 

  • Käs J, Strey H, Sackmann E (1993) Direct measurement of the wave-vector dependent bending stiffness of freely flickering actin filaments. Europhys. Lett 21:865–870

    Article  Google Scholar 

  • Käs J, Strey H, Sackmann E (1994) Direct imaging of reptation for semiflexible actin filaments. Nature 368:226–230

    Article  PubMed  Google Scholar 

  • Käs J, Strey H, Tang JX et al. (1996) F-actin, a model polymer for semiflexible chains in dilute/semidilute and liquid crystalline solutions. Biophys J 70:609–625

    Article  PubMed  Google Scholar 

  • Korn ED, Carlier MF, Pantaloni D (1987) Actin polymerization and ATP-hydrolysis. Science 238:638–644

    Article  PubMed  CAS  Google Scholar 

  • Lipowsky R, Leibler S (1986) Unbinding transition of interacting membranes. Phys Rev Lett 56:2541–2544

    Article  PubMed  CAS  Google Scholar 

  • Kroy K, Frey E (1996) Force extension relation and plateau modulus for wormlike chains. Phys Rev Lett 77:306–309

    Article  PubMed  CAS  Google Scholar 

  • MacKintosh F, Käs J, Janmey PA (1995) Elasticity of semiflexible biopolymer networks. Phys Rev Lett 75:4425–4428

    Article  PubMed  CAS  Google Scholar 

  • Müller O, Gaub H, Bärmann M, Sackmann E (1991) Viscoelastic moduli of sterically and chemically cross-linked actin networks in the dilute to semidilute regime:measurements by an oscillating disc rheometer. Macromolecules 24:3111–3120

    Article  Google Scholar 

  • Onsager L (1949) The effect of shape and interaction of colloidal particles. Ann NY Acad Sci 51:627–650

    Article  CAS  Google Scholar 

  • Pasternak C, Flicker PF, Ravid S, Spudich JA (1989) Intermolecular versus intramolecular interactions in dictyostelium myosin:possible regulation by heavy chain phosphorylation. J Cell Biol 109:203–210

    Article  PubMed  CAS  Google Scholar 

  • Piekenbrock T, Sackmann E (1992) Quasielastic light scattering study of thermal excitation of F-actin solutions and of growth kinetics of actin filaments. Biopolymers 32:1471–1489

    Article  PubMed  CAS  Google Scholar 

  • Ruddies R, Goldmann WH, Isenberg G, Sackmann E (1993) The viscoelasticity of entangled actin networks:the influence of defects and modulation by talin and vinculin. Eur Biophys J 22:309–321

    Article  PubMed  CAS  Google Scholar 

  • Sackmann E (1994) Intra- and extracellular macromolecular networks:physics and biological function. Macromol Chem Phys 195:7–28

    Article  CAS  Google Scholar 

  • Saito N, Takahashi K, Yunoki Y (1997) The statistical mechanical theory of stiff chains. J Phys Soc Jpn 22:219–225

    Article  Google Scholar 

  • Schiessel H, Blumen A (1995) Mesoscopic pictures of the sol-gel transition:ladder models and fractal networks. Macromolecules 28:4013–4019

    Article  CAS  Google Scholar 

  • Schindl M, Wallraff E, Deubzer B, White W, Gerisch G, Sackmann E (1995) Cell substrate interaction and locomotion of Dictyostelium wild-type and mutants defective in three cytoskeletal proteins:a study using quantitative reflection interference contrast microscopy. Biophys J 68:1177–1190

    Article  PubMed  CAS  Google Scholar 

  • Schleicher M, Noegel AA (1992) Dynamics of the dictyostelium cytoskeleton during Chemotaxis. New Biol 4:461–472

    PubMed  CAS  Google Scholar 

  • Schmidt C, Bärmann M, Isenberg G, Sackmann E (1989) Chain dynamics, mesh size and diffusive transport in networks of polymerized actin:a quasielastic light scattering and microfluorescence study. Macromolecules 22:3638–3648

    Article  CAS  Google Scholar 

  • Schmidt FG, Ziemann F, Sackmann E (1996) Shear field mapping in actin networks by using magnetic tweezers. J Eur Biophys 24:248–253

    Google Scholar 

  • Semenov AN, Kokhlov AR (1988) Statistical physics of liquid-crystalline polymers. Sov Phys Usp 31:988–1014

    Article  Google Scholar 

  • Stauffer D, Aharony A (1992) Introduction to percolation theory. Taylor and Francis, London

    Google Scholar 

  • Tempel M, Isenberg G, Sackmann E (1996) Temperature-induced sol-gel transition and microgel formation in α-actinin cross-linked actin networks:a rheological study. Phys Rev E 54:1802–1810

    Article  CAS  Google Scholar 

  • Wachsstock DH, Schwarz WH, Pollard TD (1993) Affinity of α-actinin for actin determines the structure and mechanical properties of actin filament gels. Biophys J 65:205–214

    Article  PubMed  CAS  Google Scholar 

  • Wachsstock DH, Schwarz WH, Pollard TD (1994) Cross-linker dynamics determine the mechanical properties of actin gels. Biophys J 66:801–809

    Article  PubMed  CAS  Google Scholar 

  • Wegner A, Savko P (1982) Fragmentation of actin filaments. Biochemistry 21:1909–1913

    Article  PubMed  CAS  Google Scholar 

  • Zaner SK, Stossel TP (1983) Physical basis of the rheological properties of F-actin. J Biol Chem 258:11004–11009

    PubMed  CAS  Google Scholar 

  • Ziemann F, Rädler J, Sackmann E (1994) Local measurement of viscoelastic moduli of entangled actin networks using an oscillating magnetic bead microrheometer. Biophys J 66:2210–2216

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Physics Department, Biophysics Laboratory, Technische Universität München, 85747, Garching, Germany

    E. Sackmann

Authors
  1. E. Sackmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Dept. of Biophysics E-22, Technical University of Munich, James-Franck-Strasse, 85747, Garching/Munich, Germany

    Gerhard Isenberg

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Sackmann, E. (1998). Viscoelasticity, Rheology and Molecular Conformational Dynamics of Entangled and Cross-Linked Actin Networks. In: Isenberg, G. (eds) Modern Optics, Electronics and High Precision Techniques in Cell Biology. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80370-3_11

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-80370-3_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-80372-7

  • Online ISBN: 978-3-642-80370-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation