Laser Microsurgery as a Tool in Single Cell Research
The patch-clamp technique is a powerful tool enabling the study of function and regulation of single channel currents and kinetics associated with the gating of ion channels. Successful application of the patch-clamp technique relies on the formation of a high resistance seal between the plasma membrane and the patch-clamp pipette. To gain access to the plasma membrane of plant cells, the cell wall must first be removed.
KeywordsCellulose Quartz Sucrose Glycerine Agar
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