Laser Microsurgery as a Tool in Single Cell Research

  • Pettie P. Booij
  • Albertus H. De Boer
Part of the Springer Lab Manual book series (SLM)

Abstract

The patch-clamp technique is a powerful tool enabling the study of function and regulation of single channel currents and kinetics associated with the gating of ion channels. Successful application of the patch-clamp technique relies on the formation of a high resistance seal between the plasma membrane and the patch-clamp pipette. To gain access to the plasma membrane of plant cells, the cell wall must first be removed.

Keywords

Cellulose Quartz Sucrose Glycerine Agar 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Pettie P. Booij
  • Albertus H. De Boer

There are no affiliations available

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