Laser Microsurgery as a Tool in Single Cell Research

  • Pettie P. Booij
  • Albertus H. De Boer
Part of the Springer Lab Manual book series (SLM)


The patch-clamp technique is a powerful tool enabling the study of function and regulation of single channel currents and kinetics associated with the gating of ion channels. Successful application of the patch-clamp technique relies on the formation of a high resistance seal between the plasma membrane and the patch-clamp pipette. To gain access to the plasma membrane of plant cells, the cell wall must first be removed.


Root Hair Bath Medium Single Channel Current Root Hair Cell Laser Microbeam 
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© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Pettie P. Booij
  • Albertus H. De Boer

There are no affiliations available

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