Abstract
The patch-clamp technique is a powerful tool enabling the study of function and regulation of single channel currents and kinetics associated with the gating of ion channels. Successful application of the patch-clamp technique relies on the formation of a high resistance seal between the plasma membrane and the patch-clamp pipette. To gain access to the plasma membrane of plant cells, the cell wall must first be removed.
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© 1998 Springer-Verlag Berlin Heidelberg
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Booij, P.P., De Boer, A.H. (1998). Laser Microsurgery as a Tool in Single Cell Research. In: Van Duijn, B., Wiltink, A. (eds) Signal Transduction — Single Cell Techniques. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80368-0_5
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DOI: https://doi.org/10.1007/978-3-642-80368-0_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48976-1
Online ISBN: 978-3-642-80368-0
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