Laser Microsurgery as a Tool in Single Cell Research
The patch-clamp technique is a powerful tool enabling the study of function and regulation of single channel currents and kinetics associated with the gating of ion channels. Successful application of the patch-clamp technique relies on the formation of a high resistance seal between the plasma membrane and the patch-clamp pipette. To gain access to the plasma membrane of plant cells, the cell wall must first be removed.
KeywordsRoot Hair Bath Medium Single Channel Current Root Hair Cell Laser Microbeam
Unable to display preview. Download preview PDF.
- Greulich KO, Weber G (1992) The light microscope on its way from an analytical to a preparative tool. J Microsc 167: 127 - 151Google Scholar
- Kurkdjian A, Leitz G, Manigault P, Harim A, Greulich KO (1993) Non-enzymatic access to the plasma membrane of Medicago root hairs by laser microsurgery. J Cell Science 105: 263 - 268Google Scholar
- Van Brussel AAN, Zaat SAJ, Canter Cremers HCJ, Wijffelman CA, Pees E, Tak T, Lugtenberg BJJ (1982) Small leguminosae as test plants for nodulation of Rhizobium leguminosarum and other rhizobia and agrobacteria harbouring a leguminosarum Sym-plasmid. Plant Sci Lett 27: 317 - 325CrossRefGoogle Scholar