Cytosolic pH and Cell Movement Measurement in Dictyostelium

  • Kei Inouye
Part of the Springer Lab Manual book series (SLM)


This chapter describes basic techniques for measuring the cytosolic pH of single motile cells by microfluorometry. A simple method of quantifying chemotactic and random cell movement is also shown.


Excitation Light Dictyostelium Discoideum Perfusion Chamber Dictyostelium Cell Fluorescence Illumination 
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© Springer-Verlag Berlin Heidelberg 1998

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  • Kei Inouye

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