An Introduction to the Working Principles of the Flow Cytometer

  • Sandor Damjanovich
  • Carlo Pieri
  • Laszlo Bene
  • Attila Jenei
  • Rezsö GáspárJr.
Part of the Springer Lab Manual book series (SLM)

Abstract

Flow cytometry is a very fast optical (spectroscopic) analysis of cells on a cell-by-cell basis. The measurement, data acquisition and analysis were made possible by an innovative application of optical spectroscopy and high tech electronics, used originally in nuclear physics. A unique characteristic of the method is the analysis of individual cells at such a high speed that biological variations and their distribution over a conveniently large cell population are accessible to statistically sound analysis within a time scale of minutes among practically physiological conditions of the cells (Shapiro 1985; Ormerod 1994).

Keywords

Anisotropy Fluores Eter Sorting Sapphire 

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References

  1. Damjanovich S, Edidin M, Szöllösi J, Trón L (1994) Mobility and proximity in biological membranes. CRC, Pearl River, NYGoogle Scholar
  2. Ormerod MG (1994) Flow cytometry: A practical approach. 2nd edn. IRL, Oxford Shapiro HM ( 1985 ) Practical flow cytometry. Alan R. Liss, New York.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Sandor Damjanovich
  • Carlo Pieri
  • Laszlo Bene
  • Attila Jenei
  • Rezsö GáspárJr.

There are no affiliations available

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