An Introduction to the Working Principles of the Flow Cytometer
Flow cytometry is a very fast optical (spectroscopic) analysis of cells on a cell-by-cell basis. The measurement, data acquisition and analysis were made possible by an innovative application of optical spectroscopy and high tech electronics, used originally in nuclear physics. A unique characteristic of the method is the analysis of individual cells at such a high speed that biological variations and their distribution over a conveniently large cell population are accessible to statistically sound analysis within a time scale of minutes among practically physiological conditions of the cells (Shapiro 1985; Ormerod 1994).
KeywordsOptical Signal Flow Chamber Scatter Signal Working Principle Ultrasound Generator
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