Autonomous Cell Labeling Using a laacZ Reporter Transgene to Produce Genetic Mosaics During Development
Analyses performed in invertebrates have revealed the central role of cell behavior for the progressive positioning and determination of cells within developing organisms (Garcia-Bellido et al. 1973; Sulston et al. 1983; Shankland 1991; Sternberg 1991). However, the study of cell behavior in organisms for which the individual sequence of embryonic cell divisions is not stereotyped remains a very complex issue and this is particularly true for vertebrates (Lawson et al. 1991; Quinlan et al. 1995). Analyses are hampered by the difficulty of labeling cells in the embryo and the lack of stable cell autonomous markers (Beddington and Lawson 1990), but also by the characteristics inherent in the prospective methods generally used.
KeywordsHydrolysis Codon Toluene Heparin Lysine
Unable to display preview. Download preview PDF.
- Beddington R, Lawson KA (1990) Clonal analysis of cell lineages. In: Copp AJ, Cockroft DL (eds) Postimplantation mammalian embryos — a practical approach. Oxford University Press, Oxford, pp 267–291Google Scholar
- Bonnerot C, Nicolas JF (1993b) Clonal analysis in the intact mouse embryo by intragenic homologous recombination. C R Acad Sci 316: 1207–1217Google Scholar
- Copp AJ (1990) Studying developmental mechanisms in intact embryos. In:. Cockroft AJC (ed) Postimplantation mammalian embryos. A practical approach Oxford University Press, Oxford, pp 293–315Google Scholar
- Franck E, Sanes JR (1991) Lineage of neurons and glia in chick dorsal root ganglia: analysis in vivo with a recombinant retrovirus. Development 111: 895–908Google Scholar
- Ledouarin H (1982) The neural crest. Developmental and cell biology. Cambridge University Press, CambridgeGoogle Scholar
- Nicolas JF, Rubenstein J (1987) Retroviral vectors. Butterworths, LondonGoogle Scholar
- Nicolas JF, Mathis L, Bonnerot C (1996) Evidence in the mouse for self-renewing stem cells in the formation of a segmented longitudinal structure, the myotome. Development 122: 1–14Google Scholar
- Sternberg PW (1991) Control of cell lineage and cell fate during nematode development. In: Bode HR (ed) Current topics in developmental biology. Academic Press, San Diego, pp 177–225Google Scholar
- Wassarman PM, Depamphilis ML (1993) Methods in enzymology: guide to techniques in mouse development. Academic Press, New YorkGoogle Scholar