Skip to main content
SpringerLink
Log in

Isolation and Identification of Contact Allergens

  • Chapter
Allergic Contact Dermatitis

Abstract

A major preventive step in the field of allergic contact dermatitis is the detection and identification of the offending allergen in the products present in the patient’s environment, both at home and at work. In many cases it is difficult to patch test properly with technical products suspected to be the cause of an allergic contact dermatitis. Irritation from a product will hide a potential allergenic activity at patch testing. In other cases, the concentration of the offending agent in a product may be too low to elicit a reaction in the limited area of a single patch test. If possible, the ingredients of a product suspected to cause contact dermatitis should be tested separately.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Stahl E (ed) (1969) Thin layer chromatography - a laboratory handbook, 2nd edn. Springer, Berlin Heidelberg New York

    Google Scholar 

  2. Jork H, Funk W, Fischer W, Wimmer H (1990) Thin layer chromatography - reagents and detection methods, vol la.VCH, Weinheim

    Google Scholar 

  3. Jork H, Funk W, Fischer W, Wimmer H (1990) Thin layer chromatography - reagents and detection methods, vol lb. VCH, Weinheim

    Google Scholar 

  4. Feigl F, Anger V (1972) Spot tests in inorganic analysis, 6th edn. Elsevier, Amsterdam

    Google Scholar 

  5. Feigl F, Anger V (1966) Spot tests in organic analysis, 7th edn. Elsevier, Amsterdam

    Google Scholar 

  6. Commission Directive 90/207/EEC amending the Second Directive 82/434/EEC on the approximation of the laws of the Member State relating to methods of analysis necessary for checking the composition of cosmetic products. Official Eur Comun 1990; no. L 108: 92–101

    Google Scholar 

  7. Fregert S, Trulsson L (1978) Simple methods for demonstration of epoxy resin of bis- phenol A type. Contact Dermatitis 4:69–72

    Article  PubMed  CAS  Google Scholar 

  8. Selim S (1977) Separation and quantitative determination of traces of carbonyl compounds as their 2,4-dinitrophenylhydrazones by high-pressure liquid chromatography. J Chromatogr 136:271–277

    Article  CAS  Google Scholar 

  9. Sainio EL, Henriks-Eckerman M-L, Kanerva L (1996) Colophony, formaldehyde and mercury in mascaras. Contact Dermatitis 34:364

    Article  PubMed  CAS  Google Scholar 

  10. Engelhardt H, Klinker R (1985) Determination of free formaldehyde in the presence of donators in cosmetics by HPLC and post-column derivation. Chromatographia 20: 559–565

    Article  CAS  Google Scholar 

  11. Bergh M, Menne T, Karlberg A-T (1994) Colophony in paper-based surgical clothing. Contact Dermatitis 31:332

    Article  PubMed  CAS  Google Scholar 

  12. Strazdins E (1989) Paper sizes and sizing. In: Zinkel DF, Rüssel J (eds) Naval stores. Production, chemistry, utilization. Pulp Chemicals Association, New York, pp 575–624

    Google Scholar 

  13. Burke RE (1989) Rosin-based printing inks. In: Zinkel DF, Rüssel J (eds) Naval stores. Production, chemistry, utilization. Pulp Chemicals Association, New York, pp 665–700

    Google Scholar 

  14. Wherry RW (1989) Other uses for rosin. In: Zinkel DF, Rüssel J (eds) Naval stores. Production, chemistry, utilization. Pulp Chemicals Association, New York, pp 701–714

    Google Scholar 

  15. Karlberg A-T, Gäfvert E, Hagelthorn G, Nilsson JLG (1988) Maleopimaric acid - a potent sensitizer in modified rosin. Contact Dermatitis 22:193–201

    Article  Google Scholar 

  16. Gäfvert E, Shao LP, Karlberg A-T, Nilsson JLG, Nilsson U (1995) Maleopimaric acid - a contact allergen in fumaric acid-modified rosin used for paper size. Nordic Pulp Paper Res J 10:139–144

    Article  Google Scholar 

  17. Gäfvert E (1994) Allergenic components in modified and unmodified rosin. Chemical characterization and studies of allergenic activity. Acta Derm Venereol Suppl (Stockh) 184

    Google Scholar 

  18. Blechinger FW (1974) Beitrag zur Dünnschichtchromatographie von Kolophonium und modifizierten Harzsäuren. Fette, Seifen, Anstrichmittel 76:275

    Article  CAS  Google Scholar 

  19. De Groot AC, Weyland JW (1988) Kathon CG: a review. J Am Acad Dermatol 18:350–358

    Article  Google Scholar 

  20. Cronin E, Hannuksela M, Lachapelle J-M, Maibach HI, Malten KE, Meneghini CL (1988) Frequency of sensitization to the preservative Kathon CG. Contact Dermatitis 18: 274–279

    Article  PubMed  CAS  Google Scholar 

  21. Frosch PJ, Schulze-Dirks A (1987) Kontaktallergie auf Kathon CG. Hautarzt 38:422–425

    PubMed  CAS  Google Scholar 

  22. Gruvberger B, Persson K, Björkner B, Bruze M, Dahlquist I, Fregert S (1986) Demonstration of Kathon CG in some commercial products. Contact Dermatitis 15:24–27

    Article  PubMed  CAS  Google Scholar 

  23. Rastogi SC (1990) Kathon CG and cosmetic products. Contact Dermatitis 22:155–160

    Article  PubMed  CAS  Google Scholar 

  24. Karlberg A-T, Bohlinder K, Boman A, Hackseil U, Hermansson J, Jacobsson S, Nilsson JLG (1988) Identification of 15-hydroperoxy-abietic acid as a contact allergen in Portuguese colophony. J Pharm Pharmacol 40:42–47

    Article  PubMed  CAS  Google Scholar 

  25. Karlberg A-T, Boman A, Hacksell U, Jacobsson S, Nilsson JLG (1988) Contact allergy to dehydroabietic acid derivatives isolated from Portuguese colophony. Contact Dermatitis 19:166–174

    Article  PubMed  CAS  Google Scholar 

  26. Krohn K, Budianto E, Flörke U, Hausen BM (1992) Untersuchung der allergenen Prinzipien aus Kolophonium: Autoxidation, Synthese und Sensibilisierung. Liebigs Ann Chem 1992:911–919

    Article  Google Scholar 

  27. Hausen BM, Börries M, Budianto E, Krohn K (1993) Contact allergy due to colophony. IX. Sensitization studies with further products isolated after oxidative degradation of resin acids and colophony. Contact Dermatitis 29:234–240

    Article  PubMed  CAS  Google Scholar 

  28. Gäfvert E, Nilsson U, Karlberg A-T, Magnusson K, Nilsson JLG (1992) Rosin allergy: identification of a dehydroabietic acid peroxide with allergenic properties. Arch Derm Res 284:409–413

    Article  PubMed  Google Scholar 

  29. Gäfvert E, Shao LP, Karlberg A-T, Nilsson U, Nilsson JLG (1994) Contact allergy to resin acid hydroperoxides. Hapten binding via free radicals and epoxides. Chem Res Toxicol 7: 260–266

    Article  PubMed  Google Scholar 

  30. Khan L, Saeed MA (1994) 13ß, 14/?-Dihydroxy-13a-isopropylabietic acid, an elicitor of contact allergy. J Pharm Sei 83:909–910

    Article  CAS  Google Scholar 

  31. Shao LP, Gäfvert E, Nilsson U, Karlberg A-T, Nilsson JLG (1995) Hydroperoxydehydro- abietic acid - a contact allergen in colophony from Pinus species. Phytochemistry 4: 853–857

    Article  Google Scholar 

  32. Holmbom B, Avela E, Pekkala S (1974) Capillary gas chromatography-mass spectrometry of resin acids in tall oil rosin. J Am Oil Chem Soc 51:397–400

    Article  CAS  Google Scholar 

  33. Ekman R (1979) Modified resin acids in the reaction zone ofFomes annosus-affected sapwood of Norway spruce. Acta Acad Abo Serie B 39/6:1–7

    Google Scholar 

  34. Ekman R, Holmbom B (1989) The wood extractives in alkaline peroxide bleaching of groundwood from Norway spruce. Nordic Pulp Paper Res J 4:188–191

    Article  CAS  Google Scholar 

  35. Karlberg A-T, Magnusson K, Nilsson U (1992) Air oxidation of d-limonene - the citrus solvent - creates potent allergens. Contact Dermatitis 26:332–340

    Article  PubMed  CAS  Google Scholar 

  36. Karlberg A-T, Shao LP, Nilsson U, Gafvert E, Nilsson JLG (1994) Hydroperoxides in oxidized d-limonene identified as potent contact allergens. Arch Derm Res 286:97–103

    Article  PubMed  CAS  Google Scholar 

  37. Karlberg A-T, Magnusson K, Nilsson U (1994) Influence of an antioxidant on the formation of allergenic compounds during autoxidation of d-limonene. Ann Occup Hyg 38: 199–207

    Article  PubMed  CAS  Google Scholar 

  38. Nilsson U, Bergh M, Shao LP, Karlberg A-T (1996) Analysis of contact allergenic compounds in oxidized d-limonene. Chromatographia 42:199–205

    Article  CAS  Google Scholar 

  39. Karlberg A-T, Dooms-Goossens A (1997) Contact allergy to oxidized d-limonene among dermatitis patients. Contract Dermatitis 36:201–206

    Article  CAS  Google Scholar 

  40. Hausen BM, Simatupang T, Bruhn G, Evers P, Koenig WA (1995) Identification of new allergenic constituents and proof of evidence for coniferyl benzoate in balsam of Peru. Am J Contact Dermatitis 6:199–208

    Article  Google Scholar 

  41. Kaniwa M-A, Momms J, Ikarashi Y, Kouima S, Nakamura A, Nakaji Y, Kurokawa Y, Kantoh H, Itoh M (1992) A method for identifying causative chemicals of allergic contact dermatitis using a combination of chemical analysis and patch testing in patients and animal groups: application to a case of rubber boot dermatitis. Contact Dermatitis 27: 166–173

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. Elisabeth Gäfvert
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ann-Therése Karlberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Clinique Dermatologique, Laboratoire de Dermatochimie associé au CNRS, Université Louis Pasteur, 1, Place de l’Hôpital, F-67091, Strasbourg Cedex, France

    Jean-Pierre Lepoittevin D.Sc.

  2. Unilever Environmental Safety Laboratory, Colworth House, MK44 1LQ, Sharbrook, Bedford, UK

    David A. Basketter

  3. Department of Dermatology, Katholieke Universiteit Leuven, University Hospital, B-3000, Leuven, Belgium

    An Goossens Ph.D.

  4. Department of Occupational Dermatology, National Institute for Working Life, S-171 84, Solna, Sweden

    Ann-Therése Karlberg Ph.D.

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Gäfvert, E., Karlberg, AT. (1998). Isolation and Identification of Contact Allergens. In: Lepoittevin, JP., Basketter, D.A., Goossens, A., Karlberg, AT. (eds) Allergic Contact Dermatitis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80331-4_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-80331-4_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-80333-8

  • Online ISBN: 978-3-642-80331-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation