Zusammenfassung
Als Grundlage für die Wirksamkeit der photodynamischen Lasertherapie (PDT) werden neben der selektiven Aufnahme bzw. Speicherung der photoaktiven Substanzen im Tumorgewebe auch frühe Effekte am Gefäßendothel postuliert. In der Mikrozirkulation wurden Vasokonstriktion, Thrombozytenaggregation, Thrombusbildung und Mikroembolisation beschrieben (2,14,16). Diese Effekte gehen mit der Freisetzung von Eicosanoiden und von Willebrandt Faktor (1,3) sowie makroskopisch mit der Schwellung von Tumorgewebe einher (7,11,17). Untersuchungen zum Einfluß der PDT auf Veränderungen des Zellvolumens fehlten bisher. Erste Untersuchungen an Tumorzellen zeigten, daß die PDT eine zeit- und dosisabhängige Schwellung von Tumorzellen induziert (10).
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Literatur
Fingar, V., Wieman, T.,Doak, K: Mechanistic studies of PDT-induced vascular damage: evidence that eicosanoids mediate this process. Int. J. Radiat. Biol., 60: 303–309, 1991.
Fingar, V., Wieman, T., Weber-Doak, K: Role of thromboxane and prostacyclin release on photodynamic therapy-induced tumor destruction. Cancer Res., 50: 2599–2603, 1990.
Foster, T., Primavera, M., Marder, V., Hilf, R., Sporn, L: Photosensitized release of von Willebrand factor from cultured human endothelial cells. Cancer Res., 51: 3261–3266, 1991.
Jaffe, E., Nachman, R., Becker, C., Minick, C: Culture of human endothelial cells derived from umbilical veine. Identification by morphologic and immunologic criteria. J. Clin. Invest., 52: 2745–2756, 1973.
Jewell, S., Bellomo, G., Thor, H., Orrenius, S: Bleb formation in hepatocytes during drug metabolism is caused by disturbances in thiol and calcium ion homeostasis. Science, 217: 1257–1259, 1982.
Kempski, O., Chaussy, L., Gross, U., Zimmer, M., Baethman, A: Volume regulation and metabolism of suspended C6 glioma cells. An in vitro model to study cytotoxic brain edema. Brain Res., 279: 217–228, 1983.
Krasner, N., Chatlani, P., Barr, H: Photodynamic therapy of tumours in gastroenterology–a review. Las.Med. Sei., 5: 233–238, 1990.
Leunig, A., Staub, F., Peters, J., Heimann, A., Csapo, C., Kempski, O., Goetz, A: Relation of early Photofrin uptake to photodynamically induced phototoxicity and changes of cell volume in different cell lines. Eur. J. Cancer, 30: 78–83, 1994.
Leunig, A., Staub, F., Peters, J., Heimann, A., Kempski, O., Goetz, A: An in vitro model to study photosensitizer uptake and photodynamic dose-response relationships of tumor cells. Res. Exp. Med., 193: 361–370, 1993.
Leunig, A., Staub, F., Peters, J., Leiderer, R., Feyh, J., Goetz, A: Die Schädigung von Tumorzellen durch die photodynamische Therapie. Laryng. Rhinol. Otol., 73: 102–107, 1994.
Li, J., Guo, Z., Jin, M., Zhao, F., Cai, W., Gao, M., Shu, M., Zou, J: Photodynamic therapy in the treatment of malignant tumours: an analysis of 540 cases. J. Photochem. Photobiol., 6: 149–155, 1990.
Malik, Z., Faraggi A: Ultrastructural damage in photosensitized endothelial cells: dependence on hematoporphyrin delivery pathways. J. Photochem. Photobiol., 14: 359–368, 1992.
Maruyama, Y: The human endothelial cell in tissue culture. Z. Zellforsch. Mikrosk. Anat, 60: 69–79, 1963.
Reed, M., Miller, F., Wieman, T., Tseng, M., Pietsch, C: The effect of photodynamic therapy on the microcirculation. J. Surg. Res., 45: 452–459, 1988.
Specht, K., Rodgers, M: Plasma membrane depolarization and calcium influx during cell injury by photodynamic action. Biochem. Biophys., 1070: 61–68, 1991.
Star, W., Marijnissen, H., van der Berg-Blok, A., Versteeg, J., Franken, K., Reinhold, H: Destruction of rat mammary tumor and normal tissue microcirculation by hematoporphyrin-derivative photoradiation observed in vivo in sandwitch observation chambers. Cancer Res., 46: 2532–2540, 1986.
Wilson, B., Mang, T., Stoll, H., Jones, C., Cooper, M., Dougherty, T: Photodynamic therapy for the treatment of basal cell carcinoma. Arch. Dermatol., 128: 1631–1636, 1992.
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© 1996 Springer-Verlag, Berlin Heidelberg
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Leunig, A., Staub, F., Plesnila, N., Peters, J., Feyh, J., Goetz, A. (1996). Aufnahme und Phototoxität von Photofrin in Humanen Endothelzellen. In: Waidelich, W., Staehler, G., Waidelich, R. (eds) Laser in der Medizin / Laser in Medicine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80264-5_14
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