PCR based analyses of epidermal cells found on adhesive tape
Adhesive tapes are commonly used by Police Forces to collect Gun Shot Residues (GSR). In a previous experiment (Torre C. and Gino S., submitted to J. Forensic Sci.) it was observed that enough DNA can be extracted, amplified and typed for the HLA-DQa locus (Saiki et al., 1986), in order to attribute with certainty a GSR stub and/or to obtain samples for DNA analyses.
KeywordsEDTA Tyrosine Agarose Bromide Electrophoresis
Unable to display preview. Download preview PDF.
- Amplitype RPM user guide, (1994). Roche Molecular System Inc., Branchburg, New Jersey.Google Scholar
- Bugawan T.L., Saiki R.K., Levenson C.H., Watson R.W. and Erlich H.A., (1988). The use of non-radioactive oligonucleotide probes to analyse enzymatically amplified DNA for prenatal diagnosis and forensic HLA typing. Bio-Technology 6, 943–947.Google Scholar
- Erlich H.A. (1989) PCR technology: principles and applications for DNA amplification. Stockton Press, New York.Google Scholar
- Innis M.A. et al., (1990). PCR protocols: a guide to methods and applications; Academic Press, San Diego.Google Scholar
- Nishimura D.Y. and Murray J.C., (1992). A tetranucleotide repeat for the F13B locus. Nucl. Acids Res. 20, 1167.Google Scholar
- Polymeropoulos M.H., Rath D.S., Xiao H. and Merril C.R., (1991). Tetranucleotide repeat polymorphism at the human tyrosine hydroxylase gene (TH). Nucl. Acids Res. 19, 3753.Google Scholar
- Sambrook J., Fritsch E.F. and Maniatis T., (1989). Molecular Cloning: a laboratory manual. Second edition, Cold Spring Harbor, New York.Google Scholar
- Yoshii T., Akiyama K., Tamura K. and Ishiyama I., (1993). PCR inhibitor: water soluble melanin, which inhibits DNA polimerases and DNases. Jpn. J. Legal Med. 47, 393–396.Google Scholar