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Multiplexed DNA Markers from Cigarette Butts in a Forensic Casework

  • F. De Stefano
  • G. Bruni
  • L. Casarino
  • M. G. Costa
  • A. Mannucci
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 6)

Abstract

As described by Hochmeister et al. [1], suitable amounts of DNA for Polymerase Chain Reaction (PCR) may be recovered from saliva cells present on cigarette butts. In such cases, detection of DNA markers may be limited both by degradation and poor amount of extracted DNA. The analysis of STR loci by fluorescent-based technology [2–3] may reduce these limitations. Moreover, coamplification of different markers in the same reaction [2–3] allows to reach more informality in defining identity of samples.

Keywords

Short Tandem Repeat Short Tandem Repeat Locus Multiplex Reaction Short Tandem Repeat Marker Forensic Casework 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Hochmeister MN, Budowle B, Jung J, Börer UV, Comey CT, Dirnhofer R (1991) PCR-based typing of DNA extracted from cigarette butts. Int J Leg Med 104:229–233CrossRefGoogle Scholar
  2. 2.
    Kimpton CP, Fisher D, Watson S, Adams M, Urquhart A, Lygo JE, Gill P (1994) Evaluation of an automated DNA profiling system employing multiplex amplification of four tetrameric STR loci. Int J Leg Med 106:302–311CrossRefGoogle Scholar
  3. 3.
    Lygo JE, Johnson PE, Holdaway DJ, Woodroffe DJ, Whitaker JP, Clayton CP, Kimpton CP, Gill P (1994) The validation of short tandem repeat (STR) loci for use in forensic casework. Int J Leg Med 107:77–89CrossRefGoogle Scholar
  4. 4.
    Mannucci A, Sullivan KM, Ivanov PL, Gill P (1994) Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin. Int J Leg Med 106:190–193CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • F. De Stefano
    • 1
  • G. Bruni
    • 1
  • L. Casarino
    • 1
  • M. G. Costa
    • 1
  • A. Mannucci
    • 1
  1. 1.Istituto di Medicina Legale dell’Università degli Studi di GenovaGenovaItaly

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