A Multiplex Amplification Approach for Simultaneous Typing of Five Loci in DNA of Ancient Basque Populations
We tried to test the commercial forensic kit worldwide known as “Polymarker” in cases of samples of very old DNA. We will try to type ancient DNA samples for five genetic systems (LDLR, GYPA, HBGG, D7S8 and GC) all of them comprised in this multiplex PCR commercial kit. Our hypothesis is that given that the amount of target ancient DNA is very small, only standardized systems already tested for succesful amplification of a few nanograms can be suitable for very scarce and damaged DNA. This kit, in the routine forensic casework, produces good results with a template DNA amount of 2–40 ngr in a final reaction volume of 100 μl.
KeywordsFinal Reaction Volume Bovine Serum Albumine Sterile Plastic Tube Typing Strip Simultaneous Typing
Unable to display preview. Download preview PDF.
- Calderón R (1994) Allelic frequency patterns in basques: Evolutionary deductions. Journal of Human Ecology 4: 37–54.Google Scholar
- Prieto L et al. (1995) Simultaneous typing of five loci in ancient DNA of three basque populations. Ancient DNA III meeting. 20–22 July. Oxford. UK. (in press).Google Scholar