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A Multiplex Amplification Approach for Simultaneous Typing of Five Loci in DNA of Ancient Basque Populations

  • Conference paper
16th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Santiago de Compostela, 12–16 September 1995

Part of the book series: Advances in Forensic Haemogenetics ((HAEMOGENETICS,volume 6))

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Abstract

We tried to test the commercial forensic kit worldwide known as “Polymarker” in cases of samples of very old DNA. We will try to type ancient DNA samples for five genetic systems (LDLR, GYPA, HBGG, D7S8 and GC) all of them comprised in this multiplex PCR commercial kit. Our hypothesis is that given that the amount of target ancient DNA is very small, only standardized systems already tested for succesful amplification of a few nanograms can be suitable for very scarce and damaged DNA. This kit, in the routine forensic casework, produces good results with a template DNA amount of 2–40 ngr in a final reaction volume of 100 μl.

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References

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Author information

Authors and Affiliations

  1. Escuela de Medicina Legal, Fac. Medicina. U. Complutense, Madrid, 28040, Spain

    Prieto, E. Arroyo, C. Asperilla & J. M. Ruiz de la Cuesta

  2. Sec. Antropología. Dep. Biol. Animal., Fac. Biol. U.de Barcelona, Spain

    A. Pérez-Pérez & D. Turbón

  3. Dep. Genética y Biol., Animal. Fac. Ciencias. U. Lejona, Bilbao, Spain

    I. Arenal

Authors
  1. Prieto
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  2. E. Arroyo
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  3. A. Pérez-Pérez
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  4. C. Asperilla
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  5. I. Arenal
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  6. J. M. Ruiz de la Cuesta
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  7. D. Turbón
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Editor information

Editors and Affiliations

  1. Institute of Legal Medicine, University of Santiago de Compostela, c/San Francisco s/n, E-15705, Santiago de Compostela, Spain

    Angel Carracedo

  2. Institut für Rechtsmedizin, Universität Münster, Von-Esmarch-Straße 86, D-48149, Münster, Germany

    Bernd Brinkmann

  3. Institute for Legal Medicine, University of Zurich, Winterthurerstraße 190, CH-8057, Zurich, Switzerland

    Walter Bär

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© 1996 Springer-Verlag Berlin Heidelberg

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Prieto et al. (1996). A Multiplex Amplification Approach for Simultaneous Typing of Five Loci in DNA of Ancient Basque Populations. In: Carracedo, A., Brinkmann, B., Bär, W. (eds) 16th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Santiago de Compostela, 12–16 September 1995. Advances in Forensic Haemogenetics, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80029-0_56

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  • DOI: https://doi.org/10.1007/978-3-642-80029-0_56

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-60492-1

  • Online ISBN: 978-3-642-80029-0

  • eBook Packages: Springer Book Archive

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