A Multiplex Amplification Approach for Simultaneous Typing of Five Loci in DNA of Ancient Basque Populations

  • Prieto
  • E. Arroyo
  • A. Pérez-Pérez
  • C. Asperilla
  • I. Arenal
  • J. M. Ruiz de la Cuesta
  • D. Turbón
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 6)

Abstract

We tried to test the commercial forensic kit worldwide known as “Polymarker” in cases of samples of very old DNA. We will try to type ancient DNA samples for five genetic systems (LDLR, GYPA, HBGG, D7S8 and GC) all of them comprised in this multiplex PCR commercial kit. Our hypothesis is that given that the amount of target ancient DNA is very small, only standardized systems already tested for succesful amplification of a few nanograms can be suitable for very scarce and damaged DNA. This kit, in the routine forensic casework, produces good results with a template DNA amount of 2–40 ngr in a final reaction volume of 100 μl.

Keywords

Phenol EDTA Agarose Chloroform Electrophoresis 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Prieto
    • 1
  • E. Arroyo
    • 1
  • A. Pérez-Pérez
    • 2
  • C. Asperilla
    • 1
  • I. Arenal
    • 3
  • J. M. Ruiz de la Cuesta
    • 1
  • D. Turbón
    • 2
  1. 1.Escuela de Medicina LegalFac. Medicina. U. ComplutenseMadridSpain
  2. 2.Sec. Antropología. Dep. Biol. Animal.Fac. Biol. U.de BarcelonaSpain
  3. 3.Dep. Genética y Biol.Animal. Fac. Ciencias. U. LejonaBilbaoSpain

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