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Automated Profiling of Multiplexed DNA Markers. An Italian Database of Four Coamplified STR Loci

  • F. De Stefano
  • L. Casarino
  • M. G. Costa
  • A. Mannucci
  • G. Bruni
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 6)

Abstract

Automated DNA profiling by fluorescent-based technology and its application on multiplex amplified polymerase chain reaction (PCR) products have been studied and validated for use in forensic laboratory routine [1–5]. The most diffused protocol provides coamplification of 4 short tandem repeat (STR) loci labelled by 2 different fluorescent dye markers and separation of PCR products by electrophoresis on an automated DNA sequencer 373A Leon (Applied Biosystems).

Keywords

Short Tandem Repeat Short Tandem Repeat Locus Tyrosine Hydroxylase Gene Diffuse Protocol Coagulation Factor Xiii 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • F. De Stefano
    • 1
  • L. Casarino
    • 1
  • M. G. Costa
    • 1
  • A. Mannucci
    • 1
  • G. Bruni
    • 1
  1. 1.Istituto di Medicina LegaleUniversità di GenovaGenovaItaly

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