Automated Profiling of Multiplexed DNA Markers. An Italian Database of Four Coamplified STR Loci
Automated DNA profiling by fluorescent-based technology and its application on multiplex amplified polymerase chain reaction (PCR) products have been studied and validated for use in forensic laboratory routine [1–5]. The most diffused protocol provides coamplification of 4 short tandem repeat (STR) loci labelled by 2 different fluorescent dye markers and separation of PCR products by electrophoresis on an automated DNA sequencer 373A Leon (Applied Biosystems).
KeywordsShort Tandem Repeat Short Tandem Repeat Locus Tyrosine Hydroxylase Gene Diffuse Protocol Coagulation Factor Xiii
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