Abstract
We are investigating the applicability of a new method called primer-extension-preamplification (PEP; Zhang et al., 1992) for forensic casework. Using standard PCR procedures the sensitivity for STR’s lies between 50–100 pg, and for AmpFLP’s between 500 pg — 1ng of genomic DNA. In the past DNA-samples containing very small amounts of template DNA could only be analyzed once. Employing PEP prior to the regular PCR we have been able to type several VNTR-loci from one PEP-sample. In order to systematically study the advantages and disadvantages of the PEP procedure, we amplified DNA-solutions in varying degrees of magnitude using random primers and analyzed aliquots of the PEP-reaction for various VNTR’s.
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References
Huber P, Holtz J (1994). Random priming and multiplex PCR with three short tandem repeats in forensic caseworks. Adv for Haemogenetics 94: 363–365
Kimpton C, Walton A, Gill P (1992). A further tetranucleotide repeat polymorphism in the vWF gene. Hum Mol Gen 1: 287
Miller S, Dykes D, Polesky H (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16: 1215
Polymeropoulos M, Raths D, Xiao H, Merril C (1991). Tetranucleotide repeat polymorphism at human coagulation factor XIII A subunit gene (F13A1). Nucleic Acids Res 19: 4036
Schmitt C, Schmutzler A, Prinz M, Staak M (1994). High sensitive DNA typing approaches for the analysis of forensic evidence: comparison of nested variable number of tandem repeats (VNTR) amplification and a short tandem repeat (STR) polymorphism. For Sci Int 66: 129–141
Zhang L, Cui X, Schmitt K, Hubert R, Navidi W, Arnheim N (1992). Whole genome amplification from a single cell: Implications for genetic analysis. Proc Natl Sci USA 92: 5847–5851
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© 1996 Springer-Verlag Berlin Heidelberg
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Baasner, A., Prinz, M., Schmitt, C. (1996). The approach of using random priming for small forensic DNA-samples. In: Carracedo, A., Brinkmann, B., Bär, W. (eds) 16th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Santiago de Compostela, 12–16 September 1995. Advances in Forensic Haemogenetics, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80029-0_43
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DOI: https://doi.org/10.1007/978-3-642-80029-0_43
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-60492-1
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