A Tetraplex PCR system for the analysis of paternity cases
Short tandem repeat loci (STR) with repeat unit lenght between 2–6 bp represent highly polymorphic markers in the human genome, that are ideal markers for genomic mapping and genetic linkage analysis and can be used for forensic and paternity applications. In contrast to the highly polymorphic VNTR loci, STR loci exhibit a limited polymorphism resulting in a reduced discrimination power. Feasability of amplification of STR loci using the polymerase chain reaction allows to multiplex several STR loci in a single PCR reaction. In combination with the use of fluorescence labelled primers employing different fluorescence dyes STR loci with overlapping or identical allele size ranges can be analyzed together in the same lane of a polyacryamid gel thus providing a rapid and sensitive method for human identification.
KeywordsAGAT Tyrosine Electrophoresis Triphosphate Rhodamine
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