A Tetraplex PCR system for the analysis of paternity cases
Short tandem repeat loci (STR) with repeat unit lenght between 2–6 bp represent highly polymorphic markers in the human genome, that are ideal markers for genomic mapping and genetic linkage analysis and can be used for forensic and paternity applications. In contrast to the highly polymorphic VNTR loci, STR loci exhibit a limited polymorphism resulting in a reduced discrimination power. Feasability of amplification of STR loci using the polymerase chain reaction allows to multiplex several STR loci in a single PCR reaction. In combination with the use of fluorescence labelled primers employing different fluorescence dyes STR loci with overlapping or identical allele size ranges can be analyzed together in the same lane of a polyacryamid gel thus providing a rapid and sensitive method for human identification.
KeywordsPolymorphic Information Content Discrimination Index Short Tandem Repeat Locus Fluorescence Label Primer Paternity Case
Unable to display preview. Download preview PDF.
- Botstein D., White R.L., Skolnick M., Davis R.W (1980) Construction of a genetic linkage map in man using restriction fragment lenght polymorphisms. Am.J.Hum.Genet. Vol 32: 182–190Google Scholar
- Polymeropoulos MH, Xiao H, Rath DS, Merril CR (1991) Tetranucleotide repeat polymorphism at the human tyrosine hydroxylase gene (TH). Nucleid Acids Research, Vol. 19: 3753Google Scholar
- Polymeropoulos MH, Xiao H, Rath DS, Merril CR (1991) Tetranucleotide repeat polymorphism at the human prostatic acid phosphatase (ACPP) gene. Nucleid Acids Research, Vol. 19, No. 17: 4792Google Scholar
- Puers C, Hammond HA, Jin L, Caskey CT, Schumm JW (1993) Identification of repeat sequence heterogeneity at the polymorphic short tandem repeat locus HUMTH01 [AATG]n and reassignement of alleles in population analysis by using a locus specific allelic ladder. Am.J.Hum.Genet. Vol 53: 953–958PubMedGoogle Scholar