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Construction and Calibration Of Allelic Ladders For The PCR-Based Systems D8S320 and AR

  • Conference paper
16th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Santiago de Compostela, 12–16 September 1995

Part of the book series: Advances in Forensic Haemogenetics ((HAEMOGENETICS,volume 6))

Abstract

Amplified fragments of STRs and AmpFlps were analyzed after electrophoretic separation and staining with ethidium bromide or silver. Exact allele determination requires comparison with allelic ladders. We construct such ladders for the tetrameric STR D8S320 and the trimeric X-linked polymorphism in the Androgen receptor gene (AR). Calibration of the ladders was performed by comparing the results of conventional separation on PAGE and subsequent silver staining with those of the ABI Sequencer in connection with the GeneScan Software 672.

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References

  • Edwards A, Hammond HA, Jin L, Caskey CT, Chakraborty R (1992): Genetic variation at five trimeric and tetrameric repeat loci in four human population groups. Genomics 12: 241–253

    Article  PubMed  CAS  Google Scholar 

  • Lubahn DB, Brown TR, Simental, JA, Higgs, HN, Migeon CJ, Wilson EM, French SF (1989): Sequence of the intron/exon junctions of the coding region of the human androgen receptor gene and identification of a point mutation in a family with complete androgen insensitivity. Proc Natl Acad Sci USA 86: 9534–9538

    Article  PubMed  CAS  Google Scholar 

  • Riley R, Nelson L, Lu J, Robertson M, Ballard L, Connoly J, Ward K (1993): Tetranucleotide polymorphism at the D8S320 locus. Hum Mol Genet 2: 1512

    Article  PubMed  CAS  Google Scholar 

  • Sleddens HFBM, Oostra BA, Brinkmann AO, Trapman J (1992): Trinucleotide repeat polymorphism in the androgen receptor gene (AR). Nucleic Acids Res 20: 1427

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Inst. of Forensic Medicine, University of Bonn, Germany

    P. Huber & J. Holtz

  2. IHF, Hamburg, Germany

    W. Schmidt

Authors
  1. P. Huber
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  2. W. Schmidt
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  3. J. Holtz
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Editor information

Editors and Affiliations

  1. Institute of Legal Medicine, University of Santiago de Compostela, c/San Francisco s/n, E-15705, Santiago de Compostela, Spain

    Angel Carracedo

  2. Institut für Rechtsmedizin, Universität Münster, Von-Esmarch-Straße 86, D-48149, Münster, Germany

    Bernd Brinkmann

  3. Institute for Legal Medicine, University of Zurich, Winterthurerstraße 190, CH-8057, Zurich, Switzerland

    Walter Bär

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© 1996 Springer-Verlag Berlin Heidelberg

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Huber, P., Schmidt, W., Holtz, J. (1996). Construction and Calibration Of Allelic Ladders For The PCR-Based Systems D8S320 and AR. In: Carracedo, A., Brinkmann, B., Bär, W. (eds) 16th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Santiago de Compostela, 12–16 September 1995. Advances in Forensic Haemogenetics, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80029-0_33

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  • DOI: https://doi.org/10.1007/978-3-642-80029-0_33

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-60492-1

  • Online ISBN: 978-3-642-80029-0

  • eBook Packages: Springer Book Archive

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