Two Highly Polymorphic VNTR Loci D5S110 (LH1) and D4S139 (Ph30): Analysis, Formal and Population Genetic Data
The experimental technique is described that enable RFLP typing with the two hypervariable VNTR loci D5S110 (LH1) and D4S139 (pH30). Population and formal genetic studies were performed using Haelll restricted DNA from 450 unrelated individuals (SW-Germany) and 35 families with 172 children (NW-Portugal). Both loci reveal more than 70 alleles ranging between 0.8 kb and 9.5 kb at D5S110 (LH1) and between 1,5 kb and 19.5 kb at D4S139 (pH30). In 172 méioses analysed, one recombination event was observed at the D4S139 locus (recombination frequency of 0.0058) and none at the locus D5S110. Allele frequency distributions were determined for each of the two loci. Heterozygosity calculation results in 93.01% for LH1 and 96.62% for ph30.
The findings show that these loci represent further genetic markers which are very informative for identity tests as well as for parentage analysis.
KeywordsRecombination Agarose Electrophoresis
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