D1S80 alleles in the Wielkopolska (Poland) population
Samples of venous blood were taken on KDTA from unrelated blood donors. PICA was extracted using phenol-chlorophorm technique. DNA content was estimated by agarose gel electrophoresis by comparison with DPA standard and adjusted to 1 ng/μl using bidestilled water. Amplification (Band et al 1992) of the D1S80 locus was carried out using 1–2 ng template and the primers described by Pudowle et al (1991). Temperature profile: denaturation 95°C, 60 s, extension 72°C, 240 s, annealing 65°C, 60 s, 27 cycles in Triothermo-block (Biometra). Separation of the fragnents was accomplished using discontinuous gel electrophoresis (Allen et al 11989). The amplified alleles were visualised by silver staining. The allelic ladder consisted of 20 alleles and was run every third electrophoretic lane. The obtained allele frequencies were compared with those for other populations, obtained using a similar technique (Skowasch et al 1992, Deka et al 1994, Kloosternan et al 1993, Miścicka-Śliwka et al 1994, Nu En Huang et al 1994, Huber and Holz 1994, Martinez-Jaretta et al 1994, Pawłowski 1995). In the cases where the results were published in the graph form, counts of alleles of individual types were calculated on the basis of allele frequencies and. the total numbers of analysed alleles.
KeywordsEurope Agarose Electrophoresis Polyacrylamide
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