Summary

Twenty Italian laboratories were involved in a collaborative study on the polymorphism of the locus D1S80. A total of 1720 blood samples of unrelated people collected in 19 Italian towns were analysed. The allelic frequencies reported by the participant laboratories were statistically homogeneous with the exception of one laboratory. More than 33 different alleles were found, ranging from 14 to over 41 repeats. Four interalleles were found and five alleles were larger than 41 repeats. A total of 146 genotypes was found. Phenotypic classes with expected frequency >4 were tested for Hardy-Weinberg equilibrium: the observed frequencies of the 51 classes did not differ significantly from the expected frequencies. No mutant allele was detected in 465 mother-child pairs.

Keywords

Agarose Electrophoresis Stratification Autochthonous 

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References

  1. Budowle B, Chakraborty R, Giusti AM, Eisemberg AJ, Allen RC (1991) Analysis of the VNTR Locus D1S80 by PCR Followed by High-Resolution PAGE. Am J Hum Genet 48:137–144.PubMedGoogle Scholar
  2. Kasai K, Nakamura Y, White R (1990) Amplification of Variable Number of Tandem Repeats (VNTR) Locus (pMCT118) by the Polymerase Chain Reaction (PCR) and its application to Forensic Science, J. Forensic Sci 35:1196–1200.PubMedGoogle Scholar
  3. Schwartz DWM, Jungl EM, Krenek OR, Mayr WR. Phenotype and allele frequencies of 4 VNTR-AMPFLP’s in an Austrian population sample. In: Advances in Forensic Haemogenetics 5 (Eds. W Bär, A Fiori & U Rossi) Springer-Verlag Berlin Heidelberg (1994) pp 578–580.Google Scholar
  4. Skowasch K, Wiegand P, Brinkmann B (1992) pMCT118 (D1S80): a new allelic ladder and an improved electrophoretic separation lead to the demonstration of 28 alleles. Int J Leg Med 105:165–168.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • G. Graziosi
    • 1
  1. 1.Department of BiologyUniversity of TriesteTriesteItaly

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