Detection of a Silent GC Allele in a Danish Mother and Child
The Group-Specific Component (GC, vitamin-D binding protein, DBP) is a polymorphic plasmaprotein still used for paternity investigations in many laboratories (theoretical chance of exclusion 31%). In a Danish case of disputed paternity inverse homozygosity was observed in a mother and her child by isoelectric focusing. Extended investigations including conventional bloodtyping systems, RFLP- and AMP/FLP-examinations were carried out. PCR/SSCP analysis of a DNA fragment containing two mutation sites specific for the alleles GC 1F, GC 1S and GC 2 in exon 11 of the GC gene was also performed.
KeywordsAlbumin Codon Polyacrylamide
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