Detection of a Silent GC Allele in a Danish Mother and Child
The Group-Specific Component (GC, vitamin-D binding protein, DBP) is a polymorphic plasmaprotein still used for paternity investigations in many laboratories (theoretical chance of exclusion 31%). In a Danish case of disputed paternity inverse homozygosity was observed in a mother and her child by isoelectric focusing. Extended investigations including conventional bloodtyping systems, RFLP- and AMP/FLP-examinations were carried out. PCR/SSCP analysis of a DNA fragment containing two mutation sites specific for the alleles GC 1F, GC 1S and GC 2 in exon 11 of the GC gene was also performed.
KeywordsAmplify Fragment Length Polymorphism Paternity Testing Amplify Fragment Length Polymorphism Technique Danish Case Major Deletion
Unable to display preview. Download preview PDF.
- Thymann M, Nellemann L J, Masumba G, Irgens-Møller L, and Morling N: Analysis of the locus D1S80 by amplified fragment length polymorphism technique (AMP-FLP). Frequency distribution in Danes. Intra and inter laboratory reproducibility of the technique. Forensic Sci Internat (1993) 60:47–56.CrossRefGoogle Scholar