Simple and Rapid Typing of STRs on an Automated DNA Sequencer

  • R. Decorte
  • J.-J. Cassiman
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 6)

Abstract

Short Tandem Repeat (STR) loci are very abundant in the human genome: di-nucleotide repeats occur approximately every 6 kb while tri- and tetra-nucleotide repeats every 300 to 500 kb (Edwards 1991). The lower size of the polymorphic region increases the sensitivity for typing of small amounts of degraded DNA which is in contrast to VNTRs. It is therefore not surprising that STR loci have become the principal polymorphic markers in forensic DNA typing. Whereas, initially the analysis of the PCR products relied on radioactive procedures, it is now possible to type STR alleles in a non-radioactive manner either by silver staining or by electrophoresis of fluorescent PCR products on an automated DNA sequencer.

Keywords

Urea EDTA Electrophoresis Polyacrylamide Dextran 

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References

  1. Decorte R, Marynen P, Cassiman JJ (1993) High-speed automated detection of PCR-amplified VNTR alleles: application to the apolipoprotein B 3’ hypervariable region. Science Tools 37:1–4Google Scholar
  2. Edwards A, Civitello A, Hammond HA, Caskey CT (1991) DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am. J. Hum. Genet. 49: 746–756PubMedGoogle Scholar
  3. Frégeau CJ, Fourney RM (1993) DNA typing with fluorescently tagged short tandem repeats: A sensitive and accurate approach to human identification. BioTechniques 15:100–119.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • R. Decorte
    • 1
  • J.-J. Cassiman
    • 1
  1. 1.Center for Human GeneticsK.U.LeuvenLeuvenBelgium

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