Simple and Rapid Typing of STRs on an Automated DNA Sequencer
Short Tandem Repeat (STR) loci are very abundant in the human genome: di-nucleotide repeats occur approximately every 6 kb while tri- and tetra-nucleotide repeats every 300 to 500 kb (Edwards 1991). The lower size of the polymorphic region increases the sensitivity for typing of small amounts of degraded DNA which is in contrast to VNTRs. It is therefore not surprising that STR loci have become the principal polymorphic markers in forensic DNA typing. Whereas, initially the analysis of the PCR products relied on radioactive procedures, it is now possible to type STR alleles in a non-radioactive manner either by silver staining or by electrophoresis of fluorescent PCR products on an automated DNA sequencer.
KeywordsUrea EDTA Electrophoresis Polyacrylamide Dextran
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