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Occurrence of Dinitrogen-fixing Bacteria and Acetylene Reduction Activity in a Rhizosphere of Five Selected Plants from Tropics

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Part of the book series: NATO ASI Series ((ASIG,volume 37))

Abstract

The aim of the papers is to characterize the occurence and distribution of dinitrogen-fixing bacteria including species of Azospirillum, Azotobacter and Clostridum in rhizosphere of the following plant species: Delonicsregia, Jecopina corsinea, Lagestroemia indica, Lawsonia inermis and Zae mays.

The samples of roots and rhizosphere soil were taken from Botanical Garden and University farm, University of Nigeria Nsukka Campus. The plant roots were studied for nitrogenase activity by acetylene reduction method. The effect of selected plants on bacteria was also determined.

Plate counts and tube dilution methods revealed the stimulation of development of bacteria in the rhizosphere of the investigated plants. The strongest stimulative effect of root systems on Azotobacter sp. was observed in the rhizosphere of Zea mays. Development of Clostridium sp. in the rhizosphere of selected plants was very irregular and dufficult to proper estimation.

All investigated root samples showed nitrogenase activity.

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References

  • Abd-El-Malek A. (1971) Isolation of anaerobic nitrogen fixers from soil. Plant and Soil 35: 432–434

    Article  Google Scholar 

  • Baldani V.L.D., Döbereiner J. (1980) Host-plant specificity in the infection of cereals with Azospirilium spp. Soil Biol Biochem. 12: 433–439

    Article  Google Scholar 

  • Becking J.J. (1981) The family Azotobacteriacea. In: The procayotes. Handbook on habitats, isolation and identification of bacteria. Vol. 1. Springer Verlag.

    Google Scholar 

  • Berkum P., Van Bohlool B.B. (1980) Evaluation of nitrogen fixation by bacteria in association with roots of tropical grasses, Microbiol. Rev. 44: 491–517

    Google Scholar 

  • Döbereiner J. (1966) Azotobacter paspali sp. uma bacteria fixadora de nitrogenio na rhizosfera de Paspalum notatum. Pesqu. Agropecu. Bas. 1:357–365

    Google Scholar 

  • Knowles R. (1977) The significance of a symbiotic nitrogen fixation by bacteria. In: A treatise on dinitrogen fixation. J.Wiley and Sons, New York

    Google Scholar 

  • Neyra C.A., Döbereiner J. (1977) Nitrogen fixation in grasses. Adv. Agronom. 29: 1–38

    Article  CAS  Google Scholar 

  • Patriquin D., Döbereiner J. (1978) Bacteria in the endorhizosphere of maize in Brazil. Can. J. Microbiol. 24: 734–742

    Article  PubMed  CAS  Google Scholar 

  • Rennie R.J. (1981) Potential use of induced mutations in improve symbioses of crop plants with N2-fixing bacteria. In: Induced mutations a tool in plant breeding. International Atomic Energy Agency, Vienna. 293–321

    Google Scholar 

  • Rodriquez E.A. (1982) Improved medium for isolation of Azospirillum species. Applied Environ. Microbiol. 44: 990–991

    Google Scholar 

  • Rovira A.D., Davey C.B. (1975) Biology of the rhizosphere. In: Plant root and its environment. Univ. Press Virginia.

    Google Scholar 

  • Umali-Garcia M., Hubbell D.H., Gaskins M.H., Dazzo B (1980) Association of Azospirillum with grass roots. Applied Environ. Microbiol. 39: 219–226

    CAS  Google Scholar 

  • Umali-Garcia M., Hubbell D.H., Gaskins M.H., Dazzo B. (1981) Absorption and mode of entry of Azospirillum brasilense to grass roots. In: Associative N2 fixation. Vol. 1, CRC Press, Boca Raton

    Google Scholar 

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© 1995 Springer-Verlag Berlin Heidelberg

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Russel, S., Ifiorah, C.C. (1995). Occurrence of Dinitrogen-fixing Bacteria and Acetylene Reduction Activity in a Rhizosphere of Five Selected Plants from Tropics. In: Fendrik, I., del Gallo, M., Vanderleyden, J., de Zamaroczy, M. (eds) Azospirillum VI and Related Microorganisms. NATO ASI Series, vol 37. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79906-8_62

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  • DOI: https://doi.org/10.1007/978-3-642-79906-8_62

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79908-2

  • Online ISBN: 978-3-642-79906-8

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