Experimental System for Creating New Type of N2-Fixing Plants
Artificial associations were established between nitrogen-fixing Azomonas or Azotobacter cells and plant tissues based on the induced carbon and energy dependency of diazotrophs on plant metabolic activity. Plant regeneration was achieved from mixed callus-bacterium associations. Light and electron microscopy was used to show the location of bacteria in intercellular spaces of callus and regenerated plant tissues. The nitrogen-fixing ability of the partnership was proved by acetylene reduction assay.
KeywordsAcetylene Reduction Assay Intercellular Region Artificial Association Endophytic Diazotrophs Forced Association
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