Influence of the Carbon Substrate on the Composition of the Exocellular Polysaccharides by Azospirillum brasilense
In the present study exocellular-polysaccharide (PS), capsular and low and high MW exo-polysaccharides produced by Azospirillum brasiliense were analysed and the influence of growth medium on the composition of all fractions was evaluated.
HPLC analyses showed that the capsular-PS contained significant amounts of glucose, galactose, rhamnose, arabinose and fucose. The approximate molar ratio of these sugars were 2:4:11:2:6. In some preparations glucuronic acid and acetyl moieties were detected.
The qualitative composition of the high MW exo-PS (EPSi) was similar to CPS but ratios were different. In particular, glucose and galactose were present in a greater amount and glucuronic acid was never detected. The low MW exo-PS (EPSs) fraction contained a higher galactose and arabinose content in comparison with the capsular fraction and no rhamnose.
KeywordsGlucuronic Acid Azospirillum Brasilense Acetyl Moiety Azospirillum Strain Azospirillum Lipoferum
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- Becking J H (1985) Pleomorphism in Azospirillum, In Azospirillum III: Genetics, Physiology, Ecology. Klingmüller W (ed) Springer-Verlag Berlin Heidelberg pp 243–262Google Scholar
- Croes C L, Moens S, Van Bastelaere E, Vanderleyden J, Michiels KW (1993) Polar flagellum mediates Azospirillum brasilense adsorption to wheat roots. J Gen Microbiol 139: 2261–2269Google Scholar
- De Troch P, Philip-Hollingsworth S, Orgambide G, Dazzo FB, Vanderleyden J (1992) Analysis of extracellular polysaccharides isolated from Azospirillum brasilense wild type and mutant strains. Symbiosis 13: 229–241Google Scholar
- De Troch P (1993) Dissertationes de agricultura: Bacterial surface polysaccharides in relation to plant interaction: a genetic and chemical study of Azospirillum brasilense. pp. 279Google Scholar
- Del Gallo M, Haegi A (1990) Characterization and quantification of exocellular polysaccharides in Azospirillum brasilense and Azospirillum lipoferum. Symbiosis 9: 155–161Google Scholar
- Dische Z (1962) General colour reactions. Methods Charbohydr Chem 1: 477–479Google Scholar
- Fani R, Bazzicalupo M, Gallori E, Giovannetti L, Ventura S, Polsinelli M (1991) Restriction fragment lenght polymorphism of Azospirillum strains. FEMS Microbiol Lett 83: 225–230Google Scholar
- Gray JX, deMaagd R, Rolfe BG, Johnston AWB, Lugtenberg JJ (1992) The role of the Rhizobium cell surface during symbiosis. In Molecular signals in plant-microbe communications Verma DPS (ed)CRC press pp 360–376Google Scholar
- Konnova SA, Baberdina IV, Makarov OE, Skvortsov IM, Ignatov VV(1990) Polysaccharide-lipid complex degradation, fractionation and monosaccharide composition of polysaccharides. Mikrobiol Zhurn 52: 40–46Google Scholar
- Konnova SA, Makarov OE, Skvortsov IM, Ignatov VV (1992) Exopolysaccharides of bacteria Azospirilium brasilense Sp 245 and Sp 107. Mikrobiol Zhurn 54: 31–42Google Scholar
- Michiels K, Vanderleiden J, Van Gool A (1989) Azospirillum-plant root associations: A review. Biol Fertil Soils 8: 356–368Google Scholar
- Michiels K W, Verreth C, Vanderleyden J (1990) Azospirillum lipoferum and Azospirillum brasilense surface polysaccharide mutants that are affected in flocculation. J Appl Bacteriol 69: 705–711Google Scholar
- Michiels K, Croes CL, Vanderleyden J (1991) Two different modes of attachment of Azospirillum brasilense Sp7 to wheat roots. J Gen Microbiol 137: 2241–2246Google Scholar
- Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111: 1–61Google Scholar
- Vanderleyden J, Costacurta A, Desair J, De Troch P, Dobbelaere S, Keijers V, Michiels K, Milcamps, Moens S, My Bekri A, Pedersen D, Van Bastelaere E, Vande Broek A, Van Dommeln A Azospirillum-Cereals: a Partnership with Future (this volume)Google Scholar
- Wicken AJ, Ayres A, Campbell LK, Knox K W (1983) Effect of growth conditions on production of Rhamnose-containing cell wall and capsular polysaccharides by strains of Lactobacillus casei subsp. rhamnosus. JBacteriol 153: 84–92Google Scholar
- Yagoda-Shagam J, Barton LL, Reed WP, Chiovetti R(1988). Fluorescein isothiocyanate-labeled lectin analysis of the surface of the nitrogen-fixing bacterium Azospirillum brasilense by flow cytometry. Appl Environ Microbiol. 54: 1831–1837Google Scholar