Influence of the Carbon Substrate on the Composition of the Exocellular Polysaccharides by Azospirillum brasilense
In the present study exocellular-polysaccharide (PS), capsular and low and high MW exo-polysaccharides produced by Azospirillum brasiliense were analysed and the influence of growth medium on the composition of all fractions was evaluated.
HPLC analyses showed that the capsular-PS contained significant amounts of glucose, galactose, rhamnose, arabinose and fucose. The approximate molar ratio of these sugars were 2:4:11:2:6. In some preparations glucuronic acid and acetyl moieties were detected.
The qualitative composition of the high MW exo-PS (EPSi) was similar to CPS but ratios were different. In particular, glucose and galactose were present in a greater amount and glucuronic acid was never detected. The low MW exo-PS (EPSs) fraction contained a higher galactose and arabinose content in comparison with the capsular fraction and no rhamnose.
KeywordsSugar Maize Polysaccharide Fractionation Fructose
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