Reduction of 4-chloronitrobenzene by Azospirillum lipoferum
The strain of Azospirillum lipoferum which reduces 4-chloronitrobenzene under aerobic and anaerobic conditions was isolated from the soil. In experiments with 14C-ring-labeled 4-chloronitrobenzene, approximately 40% of the radioactive material disappeared from the growth medium after incubation under anaerobiosis within 48 hours, but under aerobic conditions only small decrease of radioactivity in the growth medium was observed. The major two metabolites produced from 4-chloronitrobenzene in a Azospirillum culture medium were isolated and characterized. These metabolites appear to be 4-chloroacetanilide and l,3-bis(p-chlorophenyl)triazene.
KeywordsNitrite Denitrification Aniline Azobenzene Azospirillum
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- American Public Health Association (1971) “Standard Method for Examination of Water and Wastewater”. American Public Health Association, 13th ed., New YorkGoogle Scholar
- Hailas L.E., Alexander M. (1983) Microbial degradation of nitro-aromatic compounds in sewage effluent. Appl. Environ. Microbiol. 45: 1234–1241Google Scholar
- Ingraham J.L. (1981) Microbiology and genetics of denitrifiers. In: Delwich C.C. Ed. Denitrification, nitrification and atmospheric nitrous oxide. Wiley, New York, pp 45–65Google Scholar
- Neur G., Kronenberg A., Bothe H. Denitrification and nitrogen foxation by Azospirillum. III. Properties of a whcaX-Azospirillum association. Arch. Microbiol. 141: 364–370Google Scholar
- Plimmer J.R., Kearney P.C., Chisaka H., Yount J.B., Klingebiel U. (19,70) l,3-bis(3,4-dichlorophenyl)-triazene from propanil in soil. J.Agr.Food Chem. 18: 859–863Google Scholar