Abstract
In non-motile Azospirillum lipoferum 4T and A. lipoferum 4Bp, a laccase activity was characterized by several substrates and inhibitors. This laccase activity was required for the production of a brown-dark pigment, as melanin. Following random Tn5 mutagenesis in A. lipoferum 4T, several mutants affected in melanization and laccase activity were obtained. These mutations were located on the chromosome or on the cryptic 300 MDa plasmid of A. lipoferum 4T.
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Abbreviations
- SHAM:
-
Salicylhydroxamic acid
- ABTS:
-
2-2’azino-bis-(3-ethulbenzthiazoline-6-sulfonic acid)
- CTAB:
-
hexadecyltrimethylammonium bromide
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© 1995 Springer-Verlag Berlin Heidelberg
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Faure, D., Jacoud, C., Bouillant, M.L., Bally, R. (1995). Laccase activity in Azospirillum lipoferum: characteristics, melanization and Tn5 mutants. In: Fendrik, I., del Gallo, M., Vanderleyden, J., de Zamaroczy, M. (eds) Azospirillum VI and Related Microorganisms. NATO ASI Series, vol 37. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79906-8_34
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DOI: https://doi.org/10.1007/978-3-642-79906-8_34
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