Abstract
The culture fluid of A. brasilense Sp 7 was found to contain 15 mg/L of indole-3-acetic acid and 267 μg/L of abscisic acid. In the course of 10 days of growth of A. brasilense Sp 7 on root exudate medium one can trace well-defined dynamics of cytokinin activity within a period of 4 to 6 days of culture growth. It was shown that zeatin and zeatin-riboside were absent in the culture fluid of A. brasilense Sp 7. Azospirillum brasilense strains isolated from cereals from the Saratov Region, when grown in a tryptophan-supplemented medium for 3 days, produced indole-3-acetic acid in amounts of 72.6 to 108.9 μg/mg dry matter of intact cells. No close correlation was shown between the biosynthetic activity of indole-3-acetic acid and N2-fixation activity in 14 Azospirillum strains studied. A mathematical model was developed for indole-3-acetic acid synthesis by A. brasilense Sp 7 in relation to pH values, tryptophan concentration and temperature.
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References
Alekseeva IU (1975) Theoretical and experimental studies of the laws of error distribution, their classification and methods of estimating their parameters. Author’s abstract of Cand. Tech. Sci. thesis, Leningrad 20 p (In Russian)
Bar T, Okon Y (1993) Tryptophan conversion to indole-3-acetic acid via indole-3-acetamide in Azospirillum brasilense Sp7. Can J Microbiol 39: 81–86
Conard K, Stegemann B (1991) Does Azospirillum influence plant growth by cytokinins and auxins? Wennigsen Deister Germany Sept 4–7, Hannover 15–16 (Abstract)
Crozier A, Arruda P, Jasmin JM, Monteiro AMT, Sandberg G (1988) Analysis of indole-3 - acetic acid and related indoles in culture medium from Azospirillum lipoferum and Azospirillum brasilense. Appl Environ Microbiol 54: 2833–2837
Döbereiner J, Day JM (1976) Associated symbiosis in tropical grasses: characterisation of microorganisms and dinitrogen fixing sites. In: Proceedings of the 1st International Symposium on Nitrogen Fixation. Newton WH, Nymans CJ (eds) Washington State University Press, Pullman, Wash, pp 518–538
Fallik E, Okon Y, Epstein E, Goldman A, Fischer M (1989) Identification and quantification of IAA and IBA in Azospirillum brasilense — inoculated maize roots. Soil Biol Biochem 21: 147–153
Hald A (1956) Mathematical statistics with technical application. Moscow 642 p (In Russian)
Horemans S, Vlassak K (1985) Production of indole-3-acetic acid by Azospirillum brasilense. In: Azospirillum III: Genetics, Physiology, Ecology: Proceedings of the 3rd Bayreuth Azospirillum Workshop. Klingmüller W (ed) Springer-Verlag, Berlin pp 98–108
Lough TJ, Jameson PE (1990) Comparative effects of four naturally-occurring cytokinins in the Amaranthus bioassay. J Plant Physiol 136: 638–640
Maksimov VN (1980) The multifactor experiment in biology. Moscow 280 p (In Russian)
Negretskü VA, Verenchikov SP, Lozhnikova VN (1984) Identification and isolation of cytokinins from tobacco plants. Agrokhimiya 1: 89–92 (In Russian)
Nikolaeva MG, Daletskaya TV (1963) Studies on physiologically active substances in dormant seeds. Proceedings of the Botany Institute, USSR Academy of Sciences 16: 49–63 (In Russian)
Nikolaeva MG (ed) (1979) Methods of determining phytohormones and phenols in seeds. Leningrad 78 p (In Russian)
Omay SH, Schmidt WA, Martin P (1993) Indoleacetic acid production by the rhizosphere bacterium Azospirillum brasilense Cd under in vitro conditions. Can J Microbiol 39: 187–192
Palni LMS, Tay SAB, MacLeod JK (1986) GC-MS methods for cytokinins and metabolites. Gas Chromatogr. Mass Spectrom., Berlin pp 214–253
Pozdnyakova LI, Kanevskaya S V, Levanova GF, Barysheva NN, Pilipenko TYu, Bogatyrev VA, Fedorova LS (1988) Taxonomic studies on azospirilla isolated from cereals of the Saratov region. Mikrobiologiya 2: 275–278 (In Russian)
Tang YW, Bonner J (1947) The enzymatic inactivation of indoleacetic acid. I.Some characteristics of the enzyme contained in pea seedlings. Arch Biochem 13: 11–25
Tien TM, Gaskins MH, Hubbell DH (1979) Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L). Appl Environ Microbiol 37: 1016–1024
Zimmer W, Bothe H (1988) The phytohormonal interaction between Azospirillum and wheat. Plant and Soil 110: 239–247
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Iosipenko, A., Ignatov, V. (1995). Physiological aspects of phytohormone production by Azospirillum brasilense Sp 7. In: Fendrik, I., del Gallo, M., Vanderleyden, J., de Zamaroczy, M. (eds) Azospirillum VI and Related Microorganisms. NATO ASI Series, vol 37. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79906-8_33
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DOI: https://doi.org/10.1007/978-3-642-79906-8_33
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