Cloning of a Streptomyces Endoglucanase Gene in Azospirillum brasilense
The eglS gene from Streptomyces rochei A2, encoding an endoglucanase, was cloned in the conjugative vector pAF700 obtaining the 22.5 kb recombinant plasmid pCSF121. This plasmid was transferred to Escherichia coli DH1 and Azospirillum brasilense Sp6. E. coli [pCSF121] showed no endoglucanase activity, whereas A. brasilense [pCSF121] showed a low endoglucanase activity, which was cell associated. A. brasilense [pCSF121] was not able to use carboxymethyl-cellulose as carbon source. No significative difference in nitrogen fixing activity was found between A. brasilense [pCSF121] and A. brasilense [pAF700]. The different behavior of E. coli [pCSF121] and A. brasilense [pCSF121] in the expression of the eglS gene suggests that A. brasilense might recognize some Streptomyces regulative signal, i.e. for transcription, internal to the cloned fragment.
KeywordsBiomass Sugar Cellulose Agar Codon
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- Hopwood, D. A. and Kieser T. (1990). The Streptomyces genome. In: Drlica, K. and Riley, M. (Eds), The bacterial chromosome. American Society for Microbiology, Washington, D. C., pp. 147–162.Google Scholar
- Sambrook, J., Fritsch, F. E. and Maniatis, T. (1989). Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.Google Scholar
- Tarrand J.J., Krieg N.R., Dobereiner J. (1978). A taxonomic study of the Spirillum lipoferum group, with description of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can. J. Microbiol. 24: 967–980.PubMedCrossRefGoogle Scholar
- Teather, R. M. and Wood, P. J. (1982). Use of Congo red polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environm. Microbiol. 43: 777–780.Google Scholar