Abstract
A 3 kb DNA region from Azospirillum brasilense strain SPF94, located upstream of the histidine biosynthetic cluster, was cloned and analysed. The 5′ region of this sequence contained two putative ORF sharing a very high degree of sequence homology with the Escherichia coli hslUV heat-shock operon.
The region between the A. brasilense hslUV genes and the histidine operon did not contain any ORF but the analysis performed by mutual informational algorithm permitted to detected 8 tandemly repeated sequences of 21 bp. Since iterons are known to act as protein binding sites, we suppose that this sequence could play a role in regulating the histidine or thehslUV operons. Moreover the computational analysis of the DNA topology has revealed that this region is remarkably curved, a feature generally associated to protein binding sites.
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© 1995 Springer-Verlag Berlin Heidelberg
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Liò, P., Bazzicalupo, M., Grifoni, A., Mori, E., Fani, R. (1995). Cloning and Analysis of an Azospirillum brasilense Iteron and hslUV Operon Containing Region. In: Fendrik, I., del Gallo, M., Vanderleyden, J., de Zamaroczy, M. (eds) Azospirillum VI and Related Microorganisms. NATO ASI Series, vol 37. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79906-8_14
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DOI: https://doi.org/10.1007/978-3-642-79906-8_14
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