Isolation of Pure Membrane Fractions for Lipid Analysis
In order to analyse the lipid composition of different membranes, it is essential that one must be able to isolate pure membranes. As an example, this chapter describes some well-tried protocols for the isolation of different membranes from yeast cells and chloroplast membrane from plant sources.
KeywordsPermeability EDTA Vanadate Fractionation Cytosol
Unable to display preview. Download preview PDF.
- Dufourc JP, Goffeau A (1978) Solubilization by lysolecithin and purification of the plasma membrane ATPase of the yeast Schizosaccharomyces pombe. J Biol Chem 253:7026–7027Google Scholar
- Novick P, Field C, Schekman R (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 25:451–460Google Scholar
- Okorokov LA, Petrov VV (1986) Isolation of plasma membrane vesicles from the yeast Saccharomyces carlsbergensis suitable for solute transport studies. Biol Membr (USSR) 3:549–556Google Scholar
- Serrano R (1991) Transport across yeast vacuolar and plasma membranes. The molecular and cellular biology of the yeast Saccharomyces: genome dynamics, protein synthesis and energetics. I:523–585. Cold Spring Harbor Laboratory PressGoogle Scholar
- Yurina NP, Belkina GG, Piletskaya, Karapetyan NV, Odintsova MS (1991) Isolation and characterization of chloroplast DNA of Azolla pinnata. Prikl Biokhim Mikrobiol (Russian) 29:299–307Google Scholar