Transposition of Phage Mu DNA
The temperate bacteriophage Mu has been an extremely useful model system for studies on the mechanistic aspects of DNA transposition. Mu was the first element for which a soluble in vitro transposition system was established (Mizuuchi 1983). Furthermore, some of the features of the Mu system, such as the polarity of strand transfer and the chemical steps of the reaction, appear to be conserved in a wide variety of elements from bacterial transposons through mammalian viruses. The purpose of this review is to summarize the biochemical details of Mu DNA transposition which have been gleaned over recent years. Due to space limitations, an exhaustive review of the literature cannot be performed here and the reader is also referred to several other recent reviews (Pato 1989; Mizuuchi 1992a, b; Haniford and Chaconas 1992).
KeywordsStrand Transfer Integration Host Factor Synaptic Complex Strand Cleavage Integration Host Factor Protein
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