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Most transposable elements move at low frequencies and insert into many different target sites, displaying only modest or little target site-selectivity. The bacterial transposon Tn7 (Barth et al. 1976) is distinguished by its ability to insert at high frequency into a specific site called an “attachment” site, or attTn7, in the chromosomes of many bacteria, including Escherichia coli (reviewed by Craig 1989, 1991). In the absence of attTn7, for example when Tn7 inserts into plasmids, Tn7 resembles other elements and transposes to many sites at low frequency. Through both genetic and biochemical studies, it is now known that Tn7 can recognize and transpose to two different types of target sites through the action of two distinct but overlapping sets of Tn7-encoded transposition proteins.
KeywordsTransposition Reaction Simple Insertion attTn7 Site Transposition Protein Donor Backbone
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