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Qualitative RT-PCR: Amplification of Synthesized mdr-1 cDNA

  • Th. Köhler
Part of the Springer Labor Manuals book series (SLM)

Abstract

The polymerase chain reaction (PCR) is an in vitro technique allowing the amplification of specific DNA subsequences by simultaneous primer extension carried out by a heat-stable DNA polymerase added to the reaction mixture. Now performed in almost every modern laboratory, PCR has become a powerful and sensitive tool in biomedical research and one of the most widely used techniques in mRNA analysis.

Keywords

Polymerase Chain Reaction Polymerase Chain Reaction Reaction Acute Myelogenic Leukemia Polymerase Chain Reaction Buffer Polymerase Chain Reaction Protocol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Taylor GR (1991) Polymerase chain reaction: basic principles and automation. In: McPherson MJ, Quirke P, Taylor GR (eds.): PCR: A practical approach. Practical approach series, Oxford University Press, New York, pp 1 -14Google Scholar
  2. 2.
    Erlich HA (ed.) (1989) PCR technology. Principles and applications for DNA amplification. MacMillan Publishers, LondonGoogle Scholar
  3. 3.
    Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds.) (1989) PCR protocols. A guide to methods and applications. Academic Press Inc., Harcourt Brace, Jovanovich Publishers, San DiegoGoogle Scholar
  4. 4.
    Van der Bliek AM, Borst P (1989) Multidrug resistance. In: Advances in Cancer Research, Vol. 52. Academic Press, New York, pp. 165–203Google Scholar
  5. 5.
    McLean S, Hill BT (1992) An overview of membrane, cytosolic and nuclear proteins associated with the expression of resistance to multiple drugs in vitro. Biochim Biophys Acta; 1114:107–127Google Scholar
  6. 6.
    List AF, Spier C, Greer J, Wolf S, Hutter J, Dorr R, Salmon S, Futscher B, Baier M, Dalton W (1993) Phase I/II trial of cyclosporine as a chemotherapy-resistance modifier in acute leukemia. J Clin Oncol; 11:1652 -1660PubMedGoogle Scholar
  7. 7.
    Gekeler V, Frese G, Noller A, Handgretinger R, Wilisch A, Schmidt H et al. (1992) Mdrl/ P-glycoprotein, topoisomerase, and glutathione-S-transferase p gene expression in primary and relapsed state adult and childhood leukemias. Br J Cancer; 66:507–517PubMedCrossRefGoogle Scholar
  8. 8.
    Kohler T, Lafiner D, Rost A-K, Leiblein S, Remke H (in press) Polymerase chain reaction related approaches to quantitate absolute levels of mRNA coding for the multidrug resistance-associated protein and P-glycoprotein. In: Proceedings of the 2nd International Symposium “Drug resistance in Leukemia and Lymphoma”, March 6 - 8,1995, Amsterdam, “Advances in Blood Disorders” series, Harwood Academic PublishersGoogle Scholar
  9. 9.
    Morales MJ, Gottlieb DI (1993) A polymerase chain reaction-based method for detection and quantification of reporter gene expression in transient transfection assays. Anal Bio- chem; 210:188–194Google Scholar
  10. 10.
    Bebee RL, Thornton CG, Hartley JL, Rashtchian, A (1992) Contamination-free polymerase chain reaction: endonuclease cleavage and cloning of dU-PCR products. Focus; 14:53–56Google Scholar
  11. 11.
    Ruano G, Brash DE, Kidd KK (1991) PCR: the first few cycles. Amplifications; 7:1–4Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Th. Köhler

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