Qualitative RT-PCR: Amplification of Synthesized mdr-1 cDNA

  • Th. Köhler
Part of the Springer Labor Manuals book series (SLM)


The polymerase chain reaction (PCR) is an in vitro technique allowing the amplification of specific DNA subsequences by simultaneous primer extension carried out by a heat-stable DNA polymerase added to the reaction mixture. Now performed in almost every modern laboratory, PCR has become a powerful and sensitive tool in biomedical research and one of the most widely used techniques in mRNA analysis.


Polymerase Chain Reaction Polymerase Chain Reaction Reaction Acute Myelogenic Leukemia Polymerase Chain Reaction Buffer Polymerase Chain Reaction Protocol 
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© Springer-Verlag Berlin Heidelberg 1995

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  • Th. Köhler

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