Abstract
For detection or quantitation of mRNA by polymerase chain reaction, it is necessary to transcribe mRNA into copy-DNA (cDNA) by a reverse transcriptase (RT) reaction. The quality of a cDNA depends on the integrity of the messenger RNA and the fidelity of the transcription.
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Lassner, D. (1995). Synthesis of cDNA. In: Quantitation of mRNA by Polymerase Chain Reaction. Springer Labor Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79712-5_6
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DOI: https://doi.org/10.1007/978-3-642-79712-5_6
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-79714-9
Online ISBN: 978-3-642-79712-5
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