Regulation of Human B Cell Activation by Follicular Dendritic Cell and T Cell Signals
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- 1.Exponential growth of germinal center precursor cells within the primary follicles. Germinal center reaction is initiated by the rapid proliferation of about three precursor cells Kroese et al. 1987; Liu et al. 1991; Jacob end Kelsoe 1992), giving rise to a germinal center containing about 1.4 x 104 cells after 4 days of immunization (Liu at al. 1991.
A fully developed germinal center has e dark zone and a light zone. Centroblasts in the dark zone proliferate and mutate (Berek et al. 1991; Jacob et al. 1991; Koppers et al. 1993; McHeyzer-Williams et al. 1993). They give rise to non-proliferating cemrocytes in the light zone. The high-affinity centrocytes ore positively selected (Weiss et al. 1992) by antigens deposited on FIDC end differentiates 1o either memory B cells (MCHevzer-Wiumms et al. 199. 1; Schittek and Rajewsky 1992; Weiss and Rajewsky 1990) or plasma cells (Kosco et al. 1989. The low-affinity centrooytes which have failed to be selected die by apoptosio(Liu et al. 1989).
After 3-4 weeks of immunization, fully developed germinal 6entena are replaced by small follicular foci which consist of a few antigen-specific B blasts associated with immune complex networks (Liu et al. 18B1). These foci may represent the chronic stimulation of memory B cells, which are fundamental for the rnain1ainenoe of serum imrnunoglobulin (lg) level (Tew et al. 1980) and long-lived memory B cell clones (Gray and Smarvall 1988).
KeywordsGerminal Center CD40 Ligand Follicular Dendritic Cell Dark Zone Light Zone
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