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Cell Cycle Changes to the Golgi Apparatus in Animal Cells

  • Graham Warren
Conference paper
Part of the NATO ASI Series book series (volume 91)

Abstract

Dramatic changes occur to the morphology of the Golgi apparatus at the onset of mitosis in animal cells (Warren, 1985, 1993) and figure 1 provides a schematic view of this process. The compact, juxta-nuclear reticulum found in interphase cells is converted, during prophase, to several hundred discrete Golgi stacks. This is thought to occur by scission of the tubules that connect equivalent cisternae in adjacent stacks (Rambourg and Clermont, 1990; Rothman and Warren, 1994). During the middle phases of mitosis (prometaphase, metaphase and anaphase), each stack undergoes complete vesiculation to yield Golgi clusters (Lucocq et al, 1987; Lucocq and Warren, 1987). These clusters then shed vesicles which become dispersed throughout the mitotic cell cytoplasm (Lucocq et al., 1989). During telophase, these processes are reversed; clusters grow by accretion of vesicles which then fuse to reform Golgi stacks. The dispersed stacks then move to the peri-centriolar region, probably by movement along microtubules (Ho et al, 1989; Corthésy-Theulaz et al, 1992), where they undergo homotypic fusion to re-form the interphase Golgi apparatus (Lucocq et al., 1989). The end result of this stochastic process is that the original mother Golgi apparatus is equally distributed between the two daughter cells (Birky, 1983).

Keywords

Golgi Apparatus Transport Vesicle Membrane Traffic Golgi Stack Cell Cycle Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Birky C W (1983) The partitioning of cytoplasmic organelles at cell division. Int Rev Cytology 15: 49–89Google Scholar
  2. Bretscher MS , Munro S (1993) Cholesterol and the Golgi apparatus. Science 261:1–3CrossRefGoogle Scholar
  3. Collins R, Warren G (1992) Sphingolipid transport in mitotic HeLa cells. J biol Chem 267: 24906–24911PubMedGoogle Scholar
  4. Corthésy-Theulaz I, Pauloin A, Pfeffer SR (1992) Cytoplasmic dynein participates in the centrosomal localization of the Golgi complex. J Cell Biol 118: 1333–1346PubMedCrossRefGoogle Scholar
  5. Featherstone C, Griffiths G, Warren G (1985) Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells. J Cell Biol 101: 2036–2046PubMedCrossRefGoogle Scholar
  6. Ho WC, Allan VJ, van Meer G, Berger E G, Kreis T E (1989) Reclustering of scattered Golgi elements occurs along microtubules. Eur J Cell Biol 48: 250–263PubMedGoogle Scholar
  7. Kreiner T, Moore H-P (1990) Membrane traffic between secretory compartments is differentially affected during mitosis. Cell Regulation 1: 415–424PubMedGoogle Scholar
  8. Lucocq JM, Berger EG, Warren G (1989) Mitotic Golgi fragments in HeLa Cells and their role in the reassembly pathway. J Cell Biol 109: 463–474PubMedCrossRefGoogle Scholar
  9. Lucocq JM, Pryde JG, Berger EG, Warren G (1987) A mitotic form of the Golgi apparatus in HeLa cells. J Cell Biol 104: 865–874PubMedCrossRefGoogle Scholar
  10. Lucocq JM, Warren G (1987) Fragmentation and partitioning of the Golgi apparatus during mitosis in HeLa cells. EMBO J 6: 3239–3246PubMedGoogle Scholar
  11. Machamer CE (1993) Targeting and retention of Golgi membrane proteins. Curr Op Cell Biol 5: 606–612PubMedCrossRefGoogle Scholar
  12. Mackay DM, Kieckbusch R, Adamczewski JP, Warren G (1994) Cyclin A-mediated inhibition of intra-Golgi transport requires p34cdc2 . FEBS Lett 336: 549–554CrossRefGoogle Scholar
  13. Misteli T, Warren G (1994) Transport vesicles are involved in the mitotic fragmentation of Golgi stacks in a cell-free system. J Cell Biol 125: 269–282PubMedCrossRefGoogle Scholar
  14. Nilsson T, Hoe MH, Slusarewicz P, Rabouille C, Watson R, Hunte F, Watzele G, Berger EG, Warren G (1994) Kin recognition between medial Golgi enzymes in HeLa cells. EMBO J 13: 562–574PubMedGoogle Scholar
  15. Nilsson T, Slusarewicz P, Hoe M, Warren G (1993) Kin Recognition: A Model for the Retention of Golgi Enzymes. FEBS Lett 330: 1–4PubMedCrossRefGoogle Scholar
  16. Orci L, Montesano R, Meda P, Malaisse-Lagae F, Brown D, Perrelet A, Vassalli P (1981) Hetergeneous distribution of filipin-cholesterol complexes across the cisternae of the Golgi apparatus. Proc Natl Acad Sci (USA) 78: 293–297CrossRefGoogle Scholar
  17. Rambourg A, Clermont Y (1990) Three-dimensional electron microscopy: structure of the Golgi apparatus. Eur J Cell Biol 51: 189–200PubMedGoogle Scholar
  18. RothmanJE, Orci L (1992) Molecular dissection of the secretory pathway. Nature 355: 409–416PubMedCrossRefGoogle Scholar
  19. Rothman JE, Warren G (1994) Implications of the SNARE hypothesis for the specificity dynamics topology of intracellular membranes. Curr Biol 4: 220–233PubMedCrossRefGoogle Scholar
  20. Slusarewicz P, Nilsson T, Hui N, Watson R, Warren G (1994) Isolation of a matrix that binds medial Golgi enzymes. J Cell Biol 124: 405–414PubMedCrossRefGoogle Scholar
  21. Souter E, Pypaert M, Warren G (1993) The Golgi stack reassembles during telophase before arrival of proteins transported from the endoplasmic reticulum. J Cell Biol 122: 533–540PubMedCrossRefGoogle Scholar
  22. Stuart R, Mackay D, Adamczewski J, Warren G (1993) Inhibition of intra-Golgi transport in vitro by mitotic kinase. J biol Chem 268: 4050–4054PubMedGoogle Scholar
  23. Th’ng JP, Wright PS, Hamaguchi J, Lee MG, Norbury CJ, Nurse P, Bradbury EM (1990) The FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product. Cell 63: 313–24PubMedCrossRefGoogle Scholar
  24. Warren G (1985) Membrane Traffic Organelle Division. Tr Biochem Sci 10: 439–443CrossRefGoogle Scholar
  25. Warren G (1993) Membrane partitioning during cell division. Ann Rev Biochem 62: 323–348PubMedCrossRefGoogle Scholar
  26. Zieve GW, Turnbull D, Mullins JM, McIntosh JR (1980) Production of large numbers of mitotic mammalian cells by use of the reversible microtubule inhibitor nocodazole. Exp Cell Res 126: 397–405PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Graham Warren
    • 1
  1. 1.Imperial Cancer Research FundLondonUK

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