Abstract
Recent advances in studies involving the structure and dynamics of membranes have shown that fluorescently-tagged lipid probes have become versatile and, occasionally, indispensable tools in this area of research. These probes are applied in investigations as diverse as those dealing with biophysical aspects of membranes, such as lateral mobility, lipid phase transitions and phase separations (domain formation), nonbilayer formation (hexagonal phases) and lipid translocation, but also in studies of the cell biology of membranes, including membrane flow and lipid trafficking. In this brief overview, some aspects of the properties and the application of fluorescent lipid (-like) probes will be summarized. The primary focus will be on the use of nitro-benzoxadiazole-derivatized lipids (NBD-lipids).
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Hoekstra, D., Babia, T., Zegers, M., Zaal, K., Arts, E.G.J.M., Kok, J.W. (1995). Dynamical Properties of Membranes: Application of Fluorescent Lipid Analogs. In: De Lima, M.C.P., Düzgüneş, N., Hoekstra, D. (eds) Trafficking of Intracellular Membranes:. NATO ASI Series, vol 91. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79547-3_2
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DOI: https://doi.org/10.1007/978-3-642-79547-3_2
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