Skip to main content
SpringerLink
Log in
Book cover

Molecular Aspects of Oxidative Drug Metabolizing Enzymes pp 549–565Cite as

Monooxygenase Measurements as Indicators of Pollution in the Field

  • Conference paper

Part of the book series: NATO ASI Series ((ASIH,volume 90))

Abstract

Since the mid-1970’s, measurements of monooxygenase induction have been used to show the direct, sub-lethal impact of chemicals on organisms. In this lecture, I review the application of these measurements — which, in practice, are almost always of the induction of CYP 1A1, or its analogue, by a limited suite of organic compounds — to assessing the impact of water pollution on fish.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Addison RF, Edwards AJ, (1988) Hepatic microsomal monooxygenase activity in flounder Platichthvs flesus from polluted sites in Langesundfjord and from mesocosms experimentally dosed with diesel oil and copper. Mar Ecol Prog Ser 46: 51–54

    Article  CAS  Google Scholar 

  • Addison RF, Payne JF, (1986) Assessment of hepatic mixed function oxidase induction in winter flounder ( Pseudopleuronectes americanus) as a marine petroleum pollution monitoring technique, with an Appendix describing practical field measurements of MFO activity. Can Tech Rept Fish Aquat Sci no. 1505

    Google Scholar 

  • Addison RF, Willis DE, Zinck ME, (1993) Liver microsomal monooxygenase induction in winter flounder (Pseudopleuronectes americanus) from a sediment PAH gradient in Sydney Harbour, N.S. Mar Env Res, In press.

    Google Scholar 

  • Addison RF, Zinck ME, Willis DE, (1978) Induction of hepatic mixed function oxidase (MFO) enzymes in trout ( Salvelinus fontinalis) by feeding Aroclor(R) 1254 or 3-methylcholanthrene. Comp Biochem Physiol 61C: 323–325

    Article  Google Scholar 

  • Ahokas JT, Karki, NT, Oikari A and Soivio A, (1976) Mixed function mono- oxygenase of fish as an indicator of pollution of aquatic environment by industrial effluent. Bull Env Contam Toxicol 16: 270–274

    Article  CAS  Google Scholar 

  • Andersson T, Bengtsson B-E, Forlin L, Haerdig J, Larsson A, (1987) Long-term effects of bleached kraft mill effluents on carbohydrate metabolism and hepatic xenobiotic biotransformation enzymes in fish. Ecotoxicol Environ Saf 13: 53–60

    Article  PubMed  CAS  Google Scholar 

  • Bayne BL, Clarke KR, Gray JS, (Eds.) (1988) Biological effects of pollutants: results of a practical workshop. Mar Ecol Prog Ser 46: nos. 1–3

    Google Scholar 

  • Binder RL, Lech JJ, (1984) Xenobiotics in gametes of Lake Michigan lake trout ( Salvelinus namavcush) induce hepatic monooxygenase activity in their offspring. Fund Appl Toxicol 4: 1042–1054

    Article  CAS  Google Scholar 

  • Buhler DR, Rasmusson ME, (1968) The oxidation of drugs by fishes. Comp Biochem Physiol 25: 223–239

    Article  PubMed  CAS  Google Scholar 

  • Burns KA, (1976) Microsomal mixed function oxidases in an estuarine fish, Fundulus heteroclitus. and their induction as a result of environmental contamination. Comp Biochem Physiol 53B: 443–446

    Article  CAS  Google Scholar 

  • Davies JM, Bell JS, Houghton C, (1984) A comparison of the levels of hepatic aryl hydrocarbon hydroxylase in fish caught close to and distant from North Sea oil fields. Mar Env Res 14: 23–45

    Article  CAS  Google Scholar 

  • Elskus AA, Stegeman JJ, (1989) Induced cytochrome P-450 in Fundulus heteroclitus associated with environmental contamination by polychlorinated biphenyls and polynuclear aromatic hydrocarbons. Mar Env Res 27: 31–50

    Article  CAS  Google Scholar 

  • Förlin L, Andersson, T, Bengtsson, B, Harding, J, Larsson A, (1985) Effects of pulp bleach plant effluents on hepatic xenobiotic biotransformation enzymes: laboratory and field studies. Mar Env Res 17: 109–112

    Article  Google Scholar 

  • Gibaldi M, Perrier D, (Eds.) (1982). “Pharmacokinetics”, 2nd Ed., Dekker, N.Y

    Google Scholar 

  • Goksøyr A, Andersson T, Buhler DR, Stegeman JJ, Williams DE, Förlin L., (1991a) Immunochemical cross-reactivity of beta-naphthoflavone-inducible cytochrome P450 (P450IA) in liver microsomes from different fish species and rat. Fish Physiol Biochem 9: 1–13

    Google Scholar 

  • Goksøyr A, Husøy A-M, Larsen H, Klungsøyr J, Wihelmsen S, Maage A, Brevik E, Andersson T, Celander M, Pesonen P, Forlin L., (1991b) Environmental contaminants and biochemical responses in flatfish from the Hvaler Archipelago in Norway. Arch Env Contam Toxicol 21: 486–496

    Article  Google Scholar 

  • Goksøyr A, Larsen HE, Husøy AM, (1991c) Application of a cytochrome P-450 IA1-ELISA in environmental monitoring and toxicological testing of fish. Comp Biochem Physiol 100C: 157–160

    Article  Google Scholar 

  • Goksøyr A, Larsen HE, Blom S, Förlin L, (1992) Detection of cytochrome P4501A1 in North Sea dab liver and kidney. Mar Ecol Prog Ser 91: 83–88

    Article  Google Scholar 

  • Haasch ML, Kleinow KM, Lech JJ, (1988) Induction of cytochrome P-450 mRNA in rainbow trout: in vitro translation and immunodetection. Tox Appl Pharmacol 94: 246–253

    Article  CAS  Google Scholar 

  • Kezic N, Britvic S, Protic M, Simmons JE, Rijavec M, Zahn RK, Kurelec B., (1983) Activity of benzo(a)pyrene mono-oxygenase in fish from the Sava River, Yugoslavia: correlation with pollution. Sci Tot Env 27: 59–69

    Article  CAS  Google Scholar 

  • Kleinow KM, Haasch ML, Williams DE, Lech JJ, (1990) A comparison of hepatic P450 induction in rat and trout (Oncorhvnchus mvkiss): delineation of the site of resistance of fish to phenobarbital-type inducers. Comp Biochem Physiol 96C: 259–270

    Article  CAS  Google Scholar 

  • Kloepper-Sams PJ, Park SS, Gelboin HV, Stegeman JJ, (1987) Specificity and cross-reactivity of monoclonal and polyclonal antibodies against cytochrome P-450E of the marine fish scup. Arch Biochem Biophys 253: 268–278

    Article  PubMed  CAS  Google Scholar 

  • Kreamer GL, Squibb K, Gioeli D, Garte SJ, Wirgin I., (1991). Cytochrome P4501A mRNA expression in feral Hudson River tomcod. Environ Res 55: 64–78

    Article  PubMed  CAS  Google Scholar 

  • Kurelec B, Britvic S, Rijavec M, Muller WEG, Zahn RK, (1977) Benzo(a)pyrene monooxygenase induction in marine fish-molecular response to oil pollution. Mar Biol 44: 211–216

    Article  CAS  Google Scholar 

  • Lehtinen KJ, Kierkegaard A, Jakobsson E, Waendell A, (1990) Physiological effects in fish exposed to effluents from mills with six different bleaching processes. Ecotoxicol Environ Saf 19: 33–46

    Article  PubMed  CAS  Google Scholar 

  • Lindström-Seppä P, Oikari A, (1990) Biotransformation activities of feral fish in waters receiving bleached pulp mill effluents. Environ Toxicol Chem 9: 1415–1424

    Article  Google Scholar 

  • Lindström-Seppä P, Hanninen O, Hudd R, (1985) Biomonitoring of the petroleum spill in Vaasa Archipelago, Finland, by biotransformation activities in fish. In: Cytochrome P-450: Biochemistry, biophysics and induction, Eds. L. Vereczkey, and K. Magyar, pp. 357–360 Akademiai, Kiado, Budapest

    Google Scholar 

  • Lindström-Seppä P, Vuorinen PJ, Vuorinen M, Haenninen O, (1989) Effect of bleached kraft pulp mill effluent on hepatic biotransformation reaction in vendace (Coreaonus albula L.). Comp Biochem Physiol 92C: 51–54

    Google Scholar 

  • Mather Mihaich E, di Giulio RT, (1991) Oxidant, mixed-function oxidase and peroxisomal responses in channel catfish exposed to a bleached kraft mill effluent. Arch Environ Contam Toxicol 20: 391–397

    Article  Google Scholar 

  • McMaster ME, van der Kraak GJ, Portt CB, Munkittrick KR, Sibley PK, Smith IR, Dixon DG, (1991) Changes in hepatic mixed-function oxygenase (MFO) activity, plasma steroid levels and age at maturity of a white sucker (Catostomus commersoni) population exposed to bleached kraft pulp mill effluent. Aquat Toxicol 21: 199–218

    Article  CAS  Google Scholar 

  • Munkittrick KR, Portt CB, van der Kraak GJ, Smith IR, Rokosh DA, (1991) Impact of bleached kraft mill effluent on population characteristics, liver MFO activity, and serum steroid levels of a Lake Superior white sucker (Catostomus commersoni) population. Can J Fish Aquat Sci 48: 1371–1380

    Article  CAS  Google Scholar 

  • Munkittrick KR, van der Kraak GJ, McMaster ME, Portt CB, (1992) Response of hepatic MFO activity and plasma sex steroids to secondary treatment of bleached kraft pulp mill effluent and mill shutdown. Environ Toxicol Chem 11: 1427–1439

    Article  CAS  Google Scholar 

  • Nebert DW, Gelboin HV, (1968) Substrate inducible microsomal aryl hydroxylase in mammalian cell culture. J Biol Chem 243: 6242–6249

    PubMed  CAS  Google Scholar 

  • Oikari A, Lindström-Seppä P, (1990) Responses of biotransformation enzymes in fish liver: Experiments with pulpmill effluents and their components. Chemosphere 20: 1079–1085

    Google Scholar 

  • Payne JF, (1976) Field evaluation of benzo[a]pyrene hydroxylase induction as a monitor for marine pollution. Science 191: 945–946

    Article  PubMed  CAS  Google Scholar 

  • Payne JF, (1984) Mixed function oxygenases in biological monitoring programs: review of potential usage in different phyla of aquatic animals. In: “Ecotoxicological testing for the marine environment” ( G. Persoone, E. Jaspers and C. Claus, eds.) Vol. 1: 625–655. State University of Ghent and Institute for Marine Scientific Research, Bredene, Belgium

    Google Scholar 

  • Payne JF, Penrose WR, (1975) Induction of aryl hydrocarbon benzo[a]pyrene hydroxylase in fish by petroleum. Bull Environ Contam Toxicol 14: 112–116

    Article  PubMed  CAS  Google Scholar 

  • Payne JF, Bauld C, Dey AC, Kiceniuk JW and Williams U, (1984) Selectivity of mixed function oxygenase induction in flounder (Pseudopleuronectes americanus) collected at the site of the Baie Verte, Newfoundland, oil spill. Comp Biochem Physiol 79: 15–19

    Google Scholar 

  • Pequegnat WE, Wastler TA, (1980) Field bioassays for early detection of chronic impacts of chemical wastes upon marine organisms. Helg Meeres 33: 531–545

    Article  Google Scholar 

  • Renton KW, Addison RF, (1992) Hepatic microsomal monooxygenase activity and P450IA mRNA in North Sea dab (Limanda limanda) from contaminated sites. Mar Ecol Prog Ser 91: 65–69

    Article  CAS  Google Scholar 

  • Ridlington JW, Chapman DE, Boese BL, Johnson VG, Randall R, (1982) Petroleum refinery wastewater induction of the hepatic mixed-function oxidase system in Pacific Staghorn Sculpin. Arch Environ Contam Toxicol 11: 123–127

    Article  PubMed  CAS  Google Scholar 

  • Spies RB, Felton JS, Dillard L, (1982) Hepatic mixed-function oxidases in California flatfishes are increased in contaminated environments and by oil and PCB ingestion. Mar Biol 70: 117–127

    Article  CAS  Google Scholar 

  • Spies RB, Rice DW Jr, Felton J, (1988a) Effects of organic contaminants on reproduction of the starry flounder Platichthvs stellatus in San Francisco Bay. Hepatic contamination and mixed-function oxidase ( MFO) activity during the reproductive season. Mar Biol 98: 181–189

    Google Scholar 

  • Spies RB, Rice DW Jr, Felton J, (1988b) Effects of organic contaminants on reproduction of the starry flounder Platichthys stellatus in San Francisco Bay. Reproductive success of fish captured in San Francisco Bay and spawned in the laboratory. Mar Biol 98: 191–200

    Google Scholar 

  • Stegeman JJ, Kloepper-Sams, PJ, Farrington JW, (1986) Mono-oxygenase induction and chlorobiphenyls in the deep-sea fish Corvphaenoides armatus. Science 231: 1287–1289

    Article  PubMed  CAS  Google Scholar 

  • Stegeman JJ Teng FY, Snowberger EA, (1987) Induced cytochrome P450 in winter flounder (Pseudopleuronectes americanus) from coastal Massachusetts evaluated by catalytic assay and monoclonal antibody probes. Can J Fish Aquat Sci 44: 1270–1277

    Article  CAS  Google Scholar 

  • Stegeman JJ, Woodin BR, Goksøyr A, (1988) Apparent cytochrome P-450 induction as an indication of exposure to environmental chemicals in the flounder Platichthvs flesus. Mar Ecol Prog Ser 46: 55–60

    Article  CAS  Google Scholar 

  • Sulaiman N, George S, Burke MD, (1991) Assessment of sublethal pollutant impact on flounders in an industrialised estuary using hepatic biochemical indices. Mar Ecol Prog Ser 68: 207–212

    Article  CAS  Google Scholar 

  • van der Weiden MEJ, van der Kolk J, Penninks AH, Seinen W, van den Berg M, (1990) A dose/response study with 2,3,7,8-TCDD in the rainbow trout (Oncorhynchus mvkiss). Chemosphere 20: 1053–1058

    Article  Google Scholar 

  • Van Veld PA, Westbrook DJ, Woodin BR, Hale RC, Smith CL, Hugget RJ, Stegeman JJ, (1990) Induced cytochrome P450 in intestine and liver of spot (Leiostomus xanthurus) from a polycyclic aromatic hydrocarbon contaminated environment. Aquat Toxicol 17: 119–132

    Article  Google Scholar 

  • White INH, (1988) A continuous fluorimetric assay for cytochrome P-450-dependent mixed function oxidase activity using 3-cyano-7-ethoxycoumarin. Anal Biochem 172: 304–310

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Fisheries and Oceans, Ocean Chemistry Division, Institute of Ocean Sciences, P.O. Box 6000, Sidney, BC, Canada, V8L 4B2

    Richard F. Addison

Authors
  1. Richard F. Addison
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Biology, Middle East Technical University, Inönü Bulvari, Ankara, 06531, Turkey

    Emel Arinç

  2. Department of Toxicology, University of Connecticut Health Center, Farmington, Connecticut, 06032, USA

    John B. Schenkman

  3. Department of Toxicology, North Carolina State University, Raleigh, North Carolina, 27695, USA

    Ernest Hodgson

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Addison, R.F. (1995). Monooxygenase Measurements as Indicators of Pollution in the Field. In: Arinç, E., Schenkman, J.B., Hodgson, E. (eds) Molecular Aspects of Oxidative Drug Metabolizing Enzymes. NATO ASI Series, vol 90. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79528-2_27

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-79528-2_27

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79530-5

  • Online ISBN: 978-3-642-79528-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation