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Flavin-Containing Monooxygenases: Substrate Specificity and Complex Metabolic Pathways

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Molecular Aspects of Oxidative Drug Metabolizing Enzymes

Part of the book series: NATO ASI Series ((ASIH,volume 90))

Abstract

The flavin-containing monooxygenase (EC 1.14.13.8)(FMO) is located in the endoplasmic reticulum of mammalian cells and is involved in the monooxygenation of a wide variety of xenobiotics. The FMO has a similar distribution and function to many of the isozymes of cytochrome P450 (P450). Originally described as an amine oxidase (Ziegler and Mitchell, 1972), it is now known to catalyze the oxidation of many organic, and some inorganic, chemicals (Ziegler, 1990, 1991). The FAD prosthetic group first reacts with NADPH and then molecular oxygen to give rise to the enzyme-bound hydroperoxyflavin responsible for the oxidation of suitable substrates. These initial reactions occur in the absence of substrate and the enzyme exists primarily in the hydroperoxyflavin form (Poulsen and Ziegler, 1979, Beaty and Ballou, 1981a,b). A consequence of this is that substrates, with few exceptions, have the same Vmax, although Km may vary.

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Author information

Authors and Affiliations

  1. Department of Toxicology, North Carolina State University, Box 7633, Raleigh, NC, 27695, USA

    Ernest Hodgson, Bonnie L. Blake, Patricia E. Levi, Richard B. Mailman, Michael P. Lawton, Richard M. Philpot & Mary Beth Genter

  2. Brain & Development Research Center, University of North Carolina School of Medicine, Chapel Hill, NC, 27599-7250, USA

    Richard B. Mailman

  3. National Institute of Environmental Health Sciences, Research Triangle Park, NC, 27709, USA

    Michael P. Lawton & Richard M. Philpot

Authors
  1. Ernest Hodgson
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  2. Bonnie L. Blake
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  3. Patricia E. Levi
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  4. Richard B. Mailman
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  5. Michael P. Lawton
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  6. Richard M. Philpot
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  7. Mary Beth Genter
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Editor information

Editors and Affiliations

  1. Department of Biology, Middle East Technical University, Inönü Bulvari, Ankara, 06531, Turkey

    Emel Arinç

  2. Department of Toxicology, University of Connecticut Health Center, Farmington, Connecticut, 06032, USA

    John B. Schenkman

  3. Department of Toxicology, North Carolina State University, Raleigh, North Carolina, 27695, USA

    Ernest Hodgson

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© 1995 Springer-Verlag Berlin Heidelberg

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Hodgson, E. et al. (1995). Flavin-Containing Monooxygenases: Substrate Specificity and Complex Metabolic Pathways. In: Arinç, E., Schenkman, J.B., Hodgson, E. (eds) Molecular Aspects of Oxidative Drug Metabolizing Enzymes. NATO ASI Series, vol 90. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79528-2_12

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  • DOI: https://doi.org/10.1007/978-3-642-79528-2_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79530-5

  • Online ISBN: 978-3-642-79528-2

  • eBook Packages: Springer Book Archive

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