Abstract
Recently proposed reverse hybridization method is very convenient for simultaneous detection of a set nucleotide sequences in a DNA fragment of interest (1,2). The main idea of this method is immobilization of an oligonucleotide probes set on a solid support and their hybridization with the labeled DNA fragment. Here we report on an improved non-radioactive reverse hybridization method and provide an example of its application for testing point mutations responsible for β-thalassemia.
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References
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© 1995 Springer-Verlag Berlin Heidelberg
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Kozlov, I.A. et al. (1995). Genetic Analysis of Fluorescent Labeled Amplified DNA Using Series of Oligonucleotide Probes Covalently Bound to Membranes. In: Mangin, P., Ludes, B. (eds) Acta Medicinæ Legalis Vol. XLIV 1994. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79523-7_16
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DOI: https://doi.org/10.1007/978-3-642-79523-7_16
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